Enhanced phytoremediation of selenium using genetically engineered rice plants

文献类型： 外文期刊

第一作者： Li, Zhenjun

作者： Li, Zhenjun;Tian, Yongsheng;Wang, Bo;Peng, Rihe;Xu, Jing;Fu, Xiaoyan;Han, Hongjuan;Wang, Lijuan;Zhang, Wenhui;Deng, Yongdong;Wang, Yu;Gong, Zehao;Gao, Jianjie;Yao, Quanhong

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关键词： Phytoremediation; Rice; Selenium; Selenocysteine lyase; Selenocysteine methyltransferase

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.686； 五年影响因子：4.352 ）

ISSN： 0176-1617

年卷期： 2022 年 271 卷

页码：

收录情况： SCI

摘要： Selenium (Se) is a micronutrient essential for human and animal health. However, Se is toxic at high levels because the nonspecific substitution of cysteine by selenocysteine could lead to protein malfunction. In an attempt to prevent nonspecific selenocysteine incorporation into proteins, we simultaneously overexpressed the gene encoding selenocysteine lyase from Homo sapiens (HsSL), which specifically catalyzes the decomposition of selenocysteine into elemental Se-0 and alanine, and the gene encoding selenocysteine methyltransferase from Astragalus bisulcatus (AbSMT), which methylates selenocysteine into methylselenocysteine in rice. The transgenic plants showed normal growth under standard conditions. Se treatment resulted in higher levels of alanine and methylselenocysteine in transgenic plants than in wild-type plants, which indicated that this approach might have successfully redirected Se flow in the plant. Overexpression of HsSL and AbSMT in rice also endows transgenic plants with hyposensitivity to Se stress at the seed germination stage. The transgenic plants showed enhanced selenate and selenite tolerance, which was simultaneously supported by fresh weight values. Moreover, our phytoremediation assay revealed that the transgenic plants exhibited greatly improved Se elimination capabilities and accumulated about 38.5% and 128.6% more Se than wild-type plants when treated with selenate and selenite, respectively. This study offers hope that genetically modified plants could play a role in the restoration of Se-contaminated environment.

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