Supercritical CO2 extracts of propolis inhibits tumor proliferation and Enhances the immunomodulatory activity via activating the TLR4-MAPK/ NF-κB signaling pathway

文献类型： 外文期刊

第一作者： Zhu, Hequan

作者： Zhu, Hequan;Qiao, Jiangtao;Zhang, Yu;Zhang, Hongcheng;Zhang, Yu;Zhu, Hequan;Li, Chunyang;Zhu, Hequan;Qiao, Jiangtao;Zhang, Yu;Zhang, Hongcheng;Jia, Lei;El-Seedi, Hesham R.

作者机构：

关键词： Supercritical CO2 extracts from propolis (SEP); Anti-tumor; Macrophages; Mechanism; TLR4-MAPK/NF-kappa B signaling pathway; Immunomodulatory; Anti-tumor; Macrophages; Mechanism; TLR4-MAPK/NF-kappa B signaling pathway

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）

ISSN： 0963-9969

年卷期： 2024 年 196 卷

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收录情况： SCI

摘要： Propolis is a natural immunomodulator with anticancer activity. This study investigated the immunomodulatory mechanism and anti-tumor activity of supercritical CO2 extracts of propolis (SEP) in tumor-bearing immunosuppression mice. We used cyclophosphamide (CTX) to construct the immunosuppressive mice model and then inoculated them with CT26 cells to build the CT26 tumor-bearing immunosuppression mice model. Upon treatment with SEP, tumor proliferation in mice was markedly suppressed, with tumor volumes decreasing from 1881.43 mm(3) to 1049.95 mm(3) and weights reducing from 2.07 g to 1.13 g. Concurrently, the immune system recovered well, and the spleen and thymus indexes increased significantly. The total T lymphocyte (CD3(+) T cell) contents in the spleen and blood recovered from 11.88 % to 21.19 % and 15.32 % to 22.19 %, respectively. In addition, the CD4(+) /CD8(+) ratio has returned to a healthy level, 3.12 in the spleen and 5.42 in the blood. The levels of IL-1 beta, IL-6, and TNF-alpha were increased by 2.17, 2.76, and 7.15 times in the spleen, 2.76, 1.92, and 3.02 times in the serum. Moreover, the western blot results showed that SEP treatment increased the expression of toll-like receptor 4 (TLR4) and the phosphorylation of p38, extracellular signal-regulated kinase (ERK), c-Jun Nterminal kinase (JNK), and p65. These results indicated that SEP activated the immune activity of RAW 264.7 macrophages through the TLR4-mitogen-activated protein kinase (MAPK)/nuclear factor kappa B (NF-kappa B) signaling pathway to exert immunomodulatory function and inhibit tumor proliferation. This study facilitated the further application of SEP as a potential immunomodulatory and anti-tumor functional food.

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