Isolation and Activity Evaluation of Callus-Specific Promoters in Rice (Oryza sativa L.)

文献类型： 外文期刊

第一作者： Ma, Xiaojiao

作者： Ma, Xiaojiao;Wu, Chuanyin

作者机构：

关键词： callus; specific promoter; GUS reporter gene; genetic transformation; gene editing; rice

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 16 卷 5 期

页码：

收录情况： SCI

摘要： Background/Objectives: In crop genetic engineering, morphogenic genes have attracted increasing attention, given their ability to facilitate the transformation of a broad range of otherwise nontransformable cultivars. However, few callus-specific promoters have been identified to date that can be employed to avoid the adverse effects resulting from the ectopic expression of morphogenic genes on shoot regeneration and growth. Methods: A set of potential callus-specific genes were initially selected based on publicly available data. These genes were then screened using quantitative real-time polymerase chain reaction (qPCR), followed by promoter activity evaluation using a transgenic approach with the GUS gene serving as a reporter. Results: Of the 24 evaluated promoters, 12 were verified as being callus-specific using qPCR. Five genes (Os11g0295900, Os10g0207500, Os01g0300000, Os02g0252200, and Os04g0488100) were chosen, and their promoters were cloned. Based on GUS staining, the pOsTDL1B (Os10g0207500) promoter showed strong callus-specific expression, pOsEDC (Os01g0300000) was a medium-level callus-specific promoter, and pOsDLN53 (Os02g0252200) was strictly callus-specific, although its activity was low. Quantification of GUS activity indicated that all three pOsTDL1B:GUS transgenic lines exhibited strong callus specificity relative to the various tissues tested. Conclusions: A callus-specific promoter was identified that can be used to drive the expression of morphogenic genes in crop transformation.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Precise tiller angle control by manipulating TAC1 expression in rice

    作者：Yin, Tao;Tai, Yuxin;Sun, Yao;Cheng, Zixiang;Wu, Chuanyin;Sui, Yi;Sun, Yao

    关键词：genome editing; plant architecture; rice; TAC1; tiller angle

  • Regulation of tiller angle by gravity-sensing genes in rice

    作者：Yin, Tao;Sun, Yao;Tai, Yuxin;Cheng, Zixiang;Wu, Chuanyin;Sui, Yi;Sun, Yao

    关键词：Shoot gravitropism; Gravity sensing; Tiller angle; Rice; CRISPR-Cas9

  • Chromosomal translocations are a significant driver of hybrid sterility in rice

    作者：Xie, Zhenwei;Cheng, Siqi;Yu, Hao;Yu, Xiaowen;Wang, Chaolong;Yao, Bowen;Jiang, Xiaokang;Hu, Yang;Jian, Anqi;He, Xiaodong;Gao, Junwen;Chen, Minrui;Chen, Yun;Zhu, Yuantao;Liu, Shijia;Liu, Xi;Jiang, Ling;Zhao, Zhigang;Wan, Jianmin;Zheng, Hai;Wang, Jian;Ren, Yulong;Cheng, Zhijun;Lei, Cailin;Lin, Qibing;Wang, Xin;Guo, Xiuping;Wu, Chuanyin;Zhu, Shanshan;Wan, Jianmin

    关键词：hybrid sterility; pseudolinked loci; chromosomal translocation; translocated heterozygotes; pangenome

  • A comprehensive omics resource and genetic tools for functional genomics research and genetic improvement of sorghum

    作者：Chen, Chengxuan;Ge, Fengyong;Du, Huilong;Tang, Sanyuan;Zhang, Huili;Mei, Cuo;Xie, Peng;Li, Chao;Yang, Sen;Wei, Huimin;Shi, Jiayang;Zhang, Dan;Zhao, Kangxu;Yang, Dekai;Qiao, Yi;Khan, Aimal;Wodajo, Baye;Wu, Yaorong;Xia, Ran;Liang, Chengzhi;Xie, Qi;Sun, Yuanchang;Shen, Zhengwei;Yu, Feifei;Du, Huilong;Sui, Yi;Wu, Chuanyin;Chen, Chengxuan;Ge, Fengyong;Du, Huilong;Mei, Cuo;Xie, Peng;Yang, Sen;Wei, Huimin;Shi, Jiayang;Zhang, Dan;Zhao, Kangxu;Yang, Dekai;Qiao, Yi;Khan, Aimal;Wodajo, Baye;Xie, Qi;Li, Xuefeng;Luo, Zuyong;Zhang, Li

    关键词：Sorghum bicolor; genome assembly; expression atlas; mutation library; sorghum transformation

  • OsBRK1-mediated phosphorylation of OsPFN2 regulates meiotic spindle-actin assembly and rice fertility

    作者：Zheng, Hai;Zhu, Shanshan;Ren, Yulong;Xun, Ziqi;Cheng, Zhijun;Lei, Cailin;Lin, Qibing;Guo, Xiupin;Wang, Xin;Wu, Chuanyin;Wang, Haiyang;Wan, Jianmin;Zhao, Zhigang;Yu, Xiaowen;Wang, Chaolong;Yao, Bowen;Cheng, Siqi;Hu, Yang;Zhang, Shihao;Wang, Qiming;Lu, Jiayu;Xie, Zhenwei;Lei, Dekun;Jian, Anqi;Chen, Minrui;Chen, Keyi;Liu, Shijia;Liu, Xi;Tian, Yunlu;Jiang, Lin;Wan, Jianmin;Shen, Jiangfeng;Huang, Shanjin

    关键词：rice; Oryza sativa; spindle-actin assembly; spindle morphogenesis; pollen development; male sterility

  • Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet

    作者：Liu, Yang;Chen, Weiyao;Chen, Jinfeng;Cheng, Zixiang;Wu, Chuanyin;Sui, Yi;Chen, Weiyao

    关键词：broomcorn millet; CRISPR/Cas9 editing; genome assembly; herbicide resistance; transformation system

  • OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

    作者：Dong, Kun;Wu, Fuqing;Li, Shuai;Zhang, Feng;Xing, Xinxin;Jin, Xin;Luo, Sheng;Feng, Miao;Chang, Yanqi;Zhang, Shuang;Zhang, Xin;Lei, Cailin;Ren, Yulong;Zhu, Shanshan;Guo, Xiuping;Wu, Chuanyin;Lin, Qibing;Cheng, Zhijun;Wan, Jianmin;Cheng, Siqi;Miao, Rong;You, Xiaoman;Wang, Peiran;Yang, Dong-Lei;Wan, Jianmin

    关键词：protein arginine methylation; jasmonate signaling; negative feedback loop; spikelet development; adaptation to heat stress; rice

微信小程序