文献类型： 外文期刊
第一作者： Wang, Chengpeng
作者： Wang, Chengpeng;Li, Yang;Wang, Na;Yu, Qin;Gao, Junping;Zhou, Xiaofeng;Ma, Nan;Li, Yonghong
作者机构：
关键词： CRISPR; CAS9; gene editing; RhEIN2; rose
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）
ISSN： 1672-9072
年卷期： 2023 年 65 卷 4 期
页码：
收录情况： SCI
摘要： The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-related nuclease 9 (Cas9) system enables precise, simple editing of genes in many animals and plants. However, this system has not been applied to rose (Rosa hybrida) due to the genomic complexity and lack of an efficient transformation technology for this plant. Here, we established a platform for screening single-guide RNAs (sgRNAs) with high editing efficiency for CRISPR/Cas9-mediated gene editing in rose using suspension cells. We used the Arabidopsis thaliana U6-29 promoter, which showed high activity for driving sgRNA expression, to modify the CRISPR/Cas9 system. We used our highly efficient optimized CRISPR/Cas9 system to successfully edit RhEIN2, encoding an indispensable component of the ethylene signaling pathway, resulting in ethylene insensitivity in rose. Our optimized CRISPR/Cas9 system provides a powerful toolbox for functional genomics, molecular breeding, and synthetic biology in rose.
分类号：
- 相关文献
作者其他论文 更多>>
-
Decoding the fish genome opens a new era in important trait research and molecular breeding in China
作者：Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Li, Jiongtang
关键词：fish genomics; economic traits; genomic selection; genome editing; genomic breeding
-
Translatome and Transcriptome Analyses Reveal the Mechanism that Underlies the Enhancement of Salt Stress by the Small Peptide Ospep5 in Plants
作者：Wang, Jinyan;Li, Yang;Li, Mingyue;Zhang, Wenting;Ling, Xitie;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Wang, Jinyan;Li, Yang;Li, Mingyue;Zhang, Wenting;Ling, Xitie;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Wang, Jinyan;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Zheng, Zhongbing;Zhang, Baolong;Liu, Qing;Lu, Yaping;Hua, Kai
关键词：translatome; transcriptome; peptidome; small peptides; rice salt tolerance
-
Analysis of spatiotemporal land use change characteristics in the upper watershed area of the Qingshui River basin from 1990 to 2020
作者：Wang, Lei;Wang, Na;Pang, Min;Zhang, Qing;Wei, Dan;Li, Yan;An, Zhizhuang;Jin, Liang;Wang, Lei;Wang, Na;Pang, Min
关键词：Qingshui River basin; LULC; transfer matrix; redundancy analysis; driving factors
-
Application of soy protein isolate-naringenin complexes as fat replacers in low-fat cream: Based on protein conformational changes, aggregation states and interfacial adsorption behavior
作者：Zhang, Jiayu;Cheng, Tianfu;Sun, Mengya;Li, Yang;Zhang, Guofang;Hu, Zhaodong;Guo, Zengwang;Wang, Zhongjiang;Wang, Daoying;Wang, Zhongjiang
关键词：Soy protein isolate; Naringenin; Low -fat cream
-
In vitro and in vivo evaluation of thapsigargin as an antiviral agent against transmissible gastroenteritis virus
作者：Li, Yang;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Cai, Yifei;Zhang, Yue;Chen, Jianing;Fu, Yuguang;Liu, Guangliang;Liu, Yuanyuan;Liu, Guangliang;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Cai, Yifei
关键词：thapsigargin; intestinal organoids; ERS; antiviral drug; TGEV
-
Rainfall Runoff and Nitrogen Loss Characteristics on the Miyun Reservoir Slope
作者：Wang, Na;Wang, Lei;Wu, Jiajun;Pang, Min;Yang, Zhixin;Xie, Jianzhi;Wang, Na;Wang, Lei;Jin, Liang;Pang, Min;Wei, Dan;Li, Yan;Wang, Junqiang;Xu, Ting
关键词：rain intensity; slope gradient; runoff; total nitrogen
-
Transmissible gastroenteritis virus induces inflammatory responses via RIG-I/NF-κB/HIF-1α/glycolysis axis in intestinal organoids and in vivo
作者：Zhang, Yunhang;Yang, Ning;Li, Yang;Tan, Chen;Cai, Yifei;Rui, Xue;Liu, Yuanyuan;Fu, Yuguang;Liu, Guangliang;Zhang, Yunhang;Yang, Ning;Tan, Chen;Zhang, Yunhang;Yang, Ning;Li, Yang;Tan, Chen;Cai, Yifei;Rui, Xue;Liu, Yuanyuan;Liu, Guangliang;Cai, Yifei;Rui, Xue;Liu, Yuanyuan;Liu, Guangliang
关键词：TGEV; apical-out intestinal organoids; inflammation; NF-kappa B; HIF-1 alpha
