Identification and Expression Analysis of Dsx and Its Positive Transcriptional Regulation of IAG in Black Tiger Shrimp (Penaeus monodon)

文献类型： 外文期刊

第一作者： Wei, Wen-Ya

作者： Wei, Wen-Ya;Huang, Jian-Hua;Zhou, Fa-Lin;Yang, Qi-Bin;Li, Yun-Dong;Jiang, Song;Jiang, Shi-Gui;Yang, Li-Shi;Wei, Wen-Ya;Huang, Jian-Hua;Zhou, Fa-Lin;Jiang, Shi-Gui;Yang, Li-Shi;Wei, Wen-Ya;Zhou, Fa-Lin;Yang, Qi-Bin;Li, Yun-Dong;Jiang, Song;Yang, Li-Shi

作者机构：

关键词： Penaeus monodon; Dsx; insulin-like androgenic gland hormone; transcriptional regulation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 20 期

页码：

收录情况： SCI

摘要： Doublesex (Dsx) is a polymorphic transcription factor of the DMRTs family, which is involved in male sex trait development and controls sexual dimorphism at different developmental stages in arthropods. However, the transcriptional regulation of the Dsx gene is largely unknown in decapods. In this study, we reported the cDNA sequence of PmDsx in Penaeus monodon, which encodes a 257 amino acid polypeptide. It shared many similarities with Dsx homologs and has a close relationship in the phylogeny of different species. We demonstrated that the expression of the male sex differentiation gene Dsx was predominantly expressed in the P. monodon testis, and that PmDsx dsRNA injection significantly decreased the expression of the insulin-like androgenic gland hormone (IAG) and male sex-determining gene while increasing the expression of the female sex-determining gene. We also identified a 5 '-flanking region of PmIAG that had two potential cis-regulatory elements (CREs) for the PmDsx transcription. Further, the dual-luciferase reporter analysis and truncated mutagenesis revealed that PmDsx overexpression significantly promoted the transcriptional activity of the PmIAG promoter via a specific CRE. These results suggest that PmDsx is engaged in male reproductive development and positively regulates the transcription of the PmIAG by specifically binding upstream of the promoter of the PmIAG. It provides a theoretical basis for exploring the sexual regulation pathway and evolutionary dynamics of Dmrt family genes in P. monodon.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序