Enzyme-free detection of influenza A (H1N1) virus using catalytic hairpin assembly and FRET

文献类型： 外文期刊

第一作者： Zhang, Mingze

作者： Zhang, Mingze;Zhang, Mingze;Sun, He;Zhang, Zebin;Xiao, Lingyi;Han, Tingting;Yu, Songling;Hao, Zhuo;Wan, Jiayu;Zhang, Zebin;Sa, Qila;Wang, Longtao;Shen, Guangzhe

作者机构：

关键词： CHA; FRET; Enzyme-free

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2025 年 217 卷

页码：

收录情况： SCI

摘要： Precise and efficient pathogen detection is the primary line of defense for ensuring public health security. In response to this key demand, we have developed an innovative, efficient, isothermal, and enzyme-free signal amplification platform, which ingeniously integrates catalytic hairpin self-assembly (CHA) with fluorescence resonance energy transfer (FRET) technology. The specific recognition probe, which is composed of the Aptamer A and the P1/P2 chain, captures the H1N1 virus when it is present, thereby triggering the release of the P1/P2 activation chain. The released P1 and P2 chains immediately drive the two sets of CHA reactions in parallel. Within 40 min, the two sets of hairpin probes (HP) undergo cascading self-assembly to form a complementary double-chain structure. At this point, the fluorescent groups (Cy5/FAM) labeled at the end of HP are spatially close to each other, efficiently triggering the FRET effect and generating a significantly increased fluorescence signal. The detection limit of this platform is 93.75 PFU/mL, with a linear range of 93.75-12,000 PFU/mL. This enzyme-free signal amplification strategy significantly reduces the cost per reaction and has excellent stability, specificity, and accuracy. The reaction process is simple and rapid, eliminating the requirement for large-scale instruments, specialized equipment, and enzyme catalysts.

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