Characterization and expression analysis of wall-associated kinase (WAK) and WAK-like family in cotton

文献类型： 外文期刊

第一作者： Zhang, Zhiqiang

作者： Zhang, Zhiqiang;Ma, Wenyu;Ren, Zhongying;Wang, Xingxing;Zhao, Junjie;Pei, Xiaoyu;Liu, Yangai;He, Kunlun;Zhang, Fei;Li, Wei;Yang, Daigang;Ma, Xiongfeng;Huo, Wenqi

作者机构：

关键词： Wall-associated kinase; Gossypium; Evolution; Expression profiling; Abiotic stress

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 187 卷

页码：

收录情况： SCI

摘要： The wall-associated kinases (WAKs) and WAK-like kinases (WAKLs) form a group of receptor-like kinases (RLKs) with extracellular domains tightly linked to the cell wall. The WAKs/WAKLs have been known to be involved in plant growth, development, and stress responses. However, the functions of WAKs/WAKLs are less well known in cotton. In this study, 58, 66, and 99 WAK/WAKL genes were identified in Gossypium arboreum, G. raimondii, and G. hirsutum, respectively. Phylogenetic analysis showed they were classified into five groups, with two groups specific to cotton. Collinearity analysis revealed that segmental and tandem duplications resulted in expansion of the WAK/WAKL gene family in cotton. Moreover, the Ka/Ks ratios indicated this family was exposed to purifying selection pressure during evolution. The structures of the GhWAK/WAKL genes and encoded proteins suggested the functions of WAKs/WAKLs in cotton were conserved. Transient expression of four WAK/WAKL-GFP fusion constructs in Arabidopsis protoplasts indicated that they were localized on the plasma membrane. The cis -elements in the GhWAK/WAKL promoters were responsive to multiple phytohormones and abiotic stresses. Expression profiling showed that GhWAK/WAKL genes were induced by various abiotic stresses. This study provides insights into the evolution of WAK/WAKL genes and presents fundamental information for further analysis in cotton.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序