Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide
文献类型： 外文期刊
第一作者： Wu, Jieer
作者： Wu, Jieer;Yu, Yongxin;Wang, Yongjie;Wang, Yongjie;Han, Feng;Han, Feng
作者机构：
关键词：
期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.6； 五年影响因子：2.8 ）
ISSN： 0302-8933
年卷期： 2025 年 207 卷 4 期
页码：
收录情况： SCI
摘要： In our previous study, a strain of Pseudomonas composti ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 psl(+) by substituting an exogenous arabinose-inducible promoter for the native promoter of the psl gene cluster. ODT-54 psl(+) produced 50-70% more Psl EPS in FAB medium with 20 g/L arabinose induction compared to ODT-54, according to ELISA analyses. Furthermore, a 50% increase in binding to GII.6 P proteins was observed for ODT-54 psl(+). These findings confirmed the success of the construction of the ODT-54 Psl EPS overexpression strain. This work provides important experimental material and helps further explore the structure of Psl EPS from P. composti.
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