Removal of antibiotic resistance genes and mobile genetic elements in a three-stage pig manure management system: The implications of microbial community structure

文献类型： 外文期刊

第一作者： Zhao, Shuai

作者： Zhao, Shuai;Chang, Yang;Xu, Chunzhu;Liu, Jia;Sangeetha, Thangavel;Sangeetha, Thangavel;Liu, Di;Xu, Chunzhu

作者机构： Northeast Agr Univ, Coll Life Sci, Key Lab Anim Cellular & Genet Engn Heilongjiang Pr, Harbin 150030, Peoples R China;Harbin Univ, Sch Geog & Tourism, Heilongjiang Prov Key Lab Cold Reg Wetland Ecol &, Harbin 150086, Peoples R China;National Taipei Univ Technol, Res Ctr Energy Conservat New Generat Residential C, Taipei, Taiwan;Natl Taipei Univ Technol, Dept Energy & Refrigerating Air Conditioning Engn, Taipei 10608, Taiwan;Inst Anim Husb, Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

关键词： Pig manure; Antibiotic resistance genes (ARGs); Mobile genetic elements (MGEs); Bacterial community; Fungal community

期刊名称：JOURNAL OF ENVIRONMENTAL MANAGEMENT （ 2021影响因子：8.91； 五年影响因子：8.549 ）

ISSN： 0301-4797

年卷期： 2022 年 323 卷

页码：

收录情况： SCI

摘要： In this work, the removal of antibiotic resistance genes (ARGs) in the industrial-scale pig manure management system has been investigated. Additionally, the implications of mobile genetic elements (MGEs) and microbial community structure have been discussed. During the whole period of manure management, 19 ARGs and 7 MGEs were obtained from the system. The results identified that the 9 ARGs and 2 MGEs were removed from the pig manure-based materials after composting, while 5 ARGs and 2 MGEs were still remained, indicating that the ARGs/MGEs could not be removed completely as contaminants by composting. The pig farm without additional antibiotics in-feed was still faced with the risk of ARGs/MGEs from outside. Microbial community analysis illuminated that a greater decrease in the abundance of norank_f__JG30-KF-CM45, Corynebacterium, Terrispor-obacter, Truepera, Salinispora and Clostridium, was responsible for the removal of ARGs/MGEs. The genes, including tnpA-01, tnpA-02, tnpA-05, Tp614, tetQ, tetM-01, tetR-02, tetX, cfxA, floR, dfrA1 and ermF exhibited significantly positive correlation with fungal communities. Fungal community analysis verified that a remarkable decrease in the abundance of Aspergillus and Thermomyces after composting was responsible for the ARGs/MGEs removal. The results elucidated the crucial roles of the related bacterial and fungal communities in the removal of ARGs/MGEs. The compound microbial agent assisted the temperature rise of composting, thereby changing the related microbial community structure and resulting in ARGs/MGEs removal.

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