DIGOXIGENIN-LABELED CRNA PROBES FOR THE DETECTION OF 2 POTYVIRUSES INFECTING PEANUT (ARACHIS-HYPOGAEA)

文献类型： 外文期刊

第一作者： DIETZGEN, RG

作者： DIETZGEN, RG;XU, ZY;TEYCHENEY, PY

作者机构：

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 1994 年 78 卷 7 期

页码：

收录情况： SCI

摘要： Nonradioactive dot blot hybridization and chemiluminescent detection were used for the diagnosis of peanut mottle (PeMoV) and peanut stripe (PStV) potyviruses. Digoxigenin-labeled cRNA probes corresponding to the 3' terminal 1,400 (PeMoV) and 1,700 (PStV) nucleotides were transcribed from recombinant cDNA clones. Both viruses were detected in the picogram range in purified preparations and in infected peanut leaf extracts. No cross-hybridization between PStV and PeMoV was observed with either probe under conditions of high stringency. PStV cRNA probes of the complete coat protein gene and 3' untranslated region cross-hybridized with bean common mosaic virus, confirming the close relationship of these two viruses. However, a 300-nucleotide probe corresponding to the variable amino terminus of the coat protein was specific for PStV.

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