Differentiation of peanut seedborne potyviruses and cucumoviruses by RT-PCR

文献类型： 外文期刊

第一作者： Dietzgen, RG

作者： Dietzgen, RG;Callaghan, B;Higgins, CM;Birch, RG;Chen, KR;Xu, ZY

作者机构：

关键词： duplex RT-PCR;groundnut;virus diagnosis

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2001 年 85 卷 9 期

页码：

收录情况： SCI

摘要： Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine.

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