PURIFICATION OF (-)-EPIGALLOCATECHIN FROM ENZYMATIC HYDROLYSATE OF ITS GALLATE USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

文献类型： 外文期刊

第一作者： DU, QH

作者： DU, QH;LI, MJ;CHENG, Q;ZHANG, TY;ITO, Y

作者机构：

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

ISSN： 0021-9673

年卷期： 1994 年 687 卷 1 期

页码：

收录情况： SCI

摘要： Epigallocatechin gallate (EGCG) was hydrolyzed at various concentrations of tannase under pH 6.0 at 35 degrees C, and the reaction mixtures were separated by high-speed counter-current chromatography with a two-phase solvent system composed of hexane-ethyl acetate-water (1:13:20). The best results were obtained when 2 mg/ml of the enzyme buffer solution was added to 0.3 M EGCG buffers at a rate of 0.1 ml/min. Using 10 mg of the enzyme, 342 mg of epigallocatechin were obtained at a purity of 99.1%.

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