A CYP78As-small grain4-coat protein complex II pathway promotes grain size in rice

文献类型： 外文期刊

第一作者： Zhou, Chunlei

作者： Zhou, Chunlei;Lan, Jie;Miao, Rong;Liu, Xi;Zhang, Shengzhong;Pan, Tian;Wang, Jiachang;Mou, Changling;Nguyen, Thanhliem;Liu, Shijia;Jiang, Ling;Wan, Jianmin;Zhou, Chunlei;Lin, Qibing;Ren, Yulong;Feng, Miao;Wang, Xin;Luo, Sheng;Qian, Jinsheng;Luo, Wenfan;Cheng, Zhijun;Zhang, Xin;Lei, Cailin;Zhu, Shanshan;Guo, Xiuping;Wang, Jie;Zhao, Zhichao;Wan, Jianmin

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.9 ）

ISSN： 1040-4651

年卷期： 2023 年

页码：

收录情况： SCI

摘要： CYP78A, a cytochrome P450 subfamily that includes rice (Oryza sativa L.) BIG GRAIN2 (BG2, CYP78A13) and Arabidopsis thaliana KLUH (KLU, CYP78A5), generate an unknown mobile growth signal (referred to as a CYP78A-derived signal) that increases grain (seed) size. However, the mechanism by which the CYP78A pathway increases grain size remains elusive. Here, we characterized a rice small grain mutant, small grain4 (smg4), with smaller grains than its wild type due to restricted cell expansion and cell proliferation in spikelet hulls. SMG4 encodes a multidrug and toxic compound extrusion (MATE) transporter. Loss of function of SMG4 causes smaller grains while overexpressing SMG4 results in larger grains. SMG4 is mainly localized to endoplasmic reticulum (ER) exit sites (ERESs) and partially localized to the ER and Golgi. Biochemically, SMG4 interacts with coat protein complex II (COPII) components (Sar1, Sec23, and Sec24) and CYP78As (BG2, GRAIN LENGTH 3.2 [GL3.2], and BG2-LIKE 1 [BG2L1]). Genetically, SMG4 acts, at least in part, in a common pathway with Sar1 and CYP78As to regulate grain size. In summary, our findings reveal a CYP78As-SMG4-COPII regulatory pathway for grain size in rice, thus providing new insights into the molecular and genetic regulatory mechanism of grain size.

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