The mutations on the envelope glycoprotein D contribute to the enhanced neurotropism of the pseudorabies virus variant

文献类型： 外文期刊

第一作者： Wu, Hongxia

作者： Wu, Hongxia;Qi, Hansong;Wang, Bing;Li, Mingzhi;Qu, Liang;Li, Su;Luo, Yuzi;Li, Lian-Feng;Zheng, Guang-Lai;Qiu, Hua-Ji;Sun, Yuan

作者机构：

期刊名称：JOURNAL OF BIOLOGICAL CHEMISTRY （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 299 卷 11 期

页码：

收录情况： SCI

摘要： The pseudorabies virus (PRV) TJ strain, a variant of PRV, induces more severe neurological symptoms and higher mortality in piglets and mice than the PRV SC strain isolated in 1980. However, the mechanism underlying responsible for the discrepancy in virulence between these strains remains unclear. Our study investigated the differences in neurotropism between PRV TJ and PRV SC using both in vitro and in vivo models. We discovered that PRV TJ enters neural cells more efficiently than PRV SC. Furthermore, we found that PRV TJ has indistinguishable genomic DNA replication capability and axonal retrograde transport dynamics compared to the PRV SC. To gain deeper insights into the mechanisms underlying these differences, we constructed gene-interchanged chimeric virus constructs and assessed the affinity between envelope glycoprotein B, C, and D (gD) and corresponding receptors. Our findings confirmed that mutations in these envelope proteins, particularly gD, significantly contributed to the heightened attachment and penetration capabilities of PRV TJ. Our study revealed the critical importance of the gD Delta R278/P279 and gDV338A in facilitating viral invasion. Furthermore, our observations indicated that mutations in envelope proteins have a more significant impact on viral invasion than on virulence in the mouse model. Our findings provide valuable insights into the roles of natural mutations on the PRV envelope glycoproteins in cell tropism, which sheds light on the relationship between cell tropism and clinical symptoms and offers clues about viral evolution.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序