Small RNAs contribute to citrus Huanglongbing tolerance by manipulating methyl salicylate signaling and exogenous methyl salicylate primes citrus groves from emerging infection

文献类型： 外文期刊

第一作者： Cheng, Baoping

作者： Cheng, Baoping;Peng, Aitian;Cheng, Baoping;Peng, Aitian;Xu, Le;Bilal, Muhammad Saqib;Huang, Qing;Niu, Dongdong;Ma, Hongyu;Zhou, Shaoxia;Zhao, Hongwei;Xu, Le;Bilal, Muhammad Saqib;Huang, Qing;Niu, Dongdong;Ma, Hongyu;Zhou, Shaoxia;Zhao, Hongwei;Wei, Guo;Chen, Feng;Zeng, Liang;Lin, Hong;Baig, Ayesha;Wang, Xuefeng;Zou, Xiuping

作者机构：

关键词： microRNA; immunity; methyl salicylate; community immunity; gene silencing; Shatangju

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Citrus production is severely threatened by the devastating Huanglongbing (HLB) disease globally. By studying and analyzing the defensive behaviors of an HLB-tolerant citrus cultivar 'Shatangju', we discovered that citrus can sense Candidatus Liberibacter asiaticus (CLas) infection and induce immune responses against HLB, which can be further strengthened by both endogenously produced and exogenously applied methyl salicylate (MeSA). This immune circuit is turned on by an miR2977-SAMT (encoding a citrus Salicylate [SA] O-methyltransferase) cascade, by which CLas infection leads to more in planta MeSA production and aerial emission. We provided both transgenic and multi-year trail evidences that MeSA is an effective community immune signal. Ambient MeSA accumulation and foliage application can effectively induce defense gene expression and significantly boost citrus performance. We also found that miRNAs are battle fields between citrus and CLas, and about 30% of the differential gene expression upon CLas infection are regulated by miRNAs. Furthermore, CLas hijacks host key processes by manipulating key citrus miRNAs, and citrus employs miRNAs that coordinately regulate defense-related genes. Based on our results, we proposed that miRNAs and associated components are key targets for engineering or breeding resistant citrus varieties. We anticipate that MeSA-based management, either induced expression or external application, would be a promising tool for HLB control.

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