Comparative Transcriptome and Hormone Analysis of Mature Leaves and New Shoots in Tea Cuttings (Camellia sinensis) among Three Cultivars with Different Rooting Abilities

文献类型： 外文期刊

第一作者： Fan, Kai

作者： Fan, Kai;Shi, Yujie;Luo, Danni;Qian, Wenjun;Wang, Yu;Shen, Jiazhi;Ding, Zhaotang;Ding, Shibo

作者机构：

关键词： Camellia sinensis; Adventitious root formation; IAA; WGCNA; AUX; LAX

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN： 0721-7595

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收录情况： SCI

摘要： Adventitious root (AR) formation is essential for the successful propagation of Camellia sinensis. Endogenous phytohormones play an important role in this process, which is strongly dependent on genotype. However, the molecular mechanism underlying this varietal difference is not clearly understood. Here, we combined metabolomic and transcriptomic analyses of new shoots and mature leaves among three cultivars with different rooting abilities and then performed WGCNA to mine key regulators during AR formation. Hormone analysis showed that the easy-to-root cultivar BHZ had a higher indole-3-acetic acid (IAA), salicylic acid, abscisic acid, and jasmonic acids content in new shoots than the other cultivars. A crosstalk regulatory network including 14 coexpression modules was successfully constructed. Among them, a key module which had a highly positive correlation with IAA was identified. The KEGG analysis showed that related genes were enriched in "Plant hormone signal transduction". Finally, the auxin influx carrier AUX1/LAX family was considered a hub gene in AR formation. Gene expression analysis showed that the expression of CsLAXs was higher in BHZ than other cultivars, and response to different abiotic stresses. Taken together, the present omics-level WGCNA analysis provides novel insights into the molecular mechanism underlying hormonal regulation in adventitious roots formation, and the identified hub genes provide gene resources for improving AR formation.

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