Cloning and Characterization of the Coding Sequences of the 1Ay High Molecular Weight Glutenin Subunit Genes from Triticum urartu

文献类型： 外文期刊

第一作者： Bai, JR

作者： Bai, JR;Jia, X;Liu, KF;Wang, DW

作者机构：

关键词： high molecular weight glutenin subunit;1Ay;triticum urartu;common wheat

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Using degenerate oligonucleotide primers and genomic PCR reactions, the complete coding region sequences of two 1Ay high molecular weight (HMW) glutenin subunit genes were amplified from Triticum urartu accessions that showed differential expression of the 1Ay HMW glutenin subunits in their seeds. The coding sequence amplified from the accession that expressed the 1Ay gene (Tu1Ay-e) was highly homologous to that of known y type HMW glutenin subunit genes. Consequently, the primary structure of the protein translated from the coding sequence of Tu1Ay-e was identical to that of previously published y type subunits. Bacterial expression of the coding sequence of Tu1Ay-e produced a polypeptide identical to the 1Ay subunit extracted from seeds, indicating that the cloned sequence was an accurate representation of the original coding region of Tu1Ay-e. In contrast, the coding region sequence amplified from the accession that did not express 1Ay subunit contained three in-frame premature stop codons. Based on past findings on silenced HMW glutenin subunit genes, we conclude that the presence of in-frame premature stop codon(s) is an important feature of the silenced 1Ay gene (Tu1Ay-s) in T. urartu. The potential value of the active 1Ay gene in improving the end use quality of common wheat and the mechanism underlying 1Ay gene silencing are discussed.

分类号： Q94

  • 相关文献

[1]Testing and modelling the potential of three diploid plants in Poaceae as a new pathosystem to investigate the interactions between cereal hosts and cereal cyst nematode (Heterodera avenae). Wu, D. Q.,Cui, J. K.,Huang, W. K.,Peng, H.,Peng, D. L..

[2]Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat. Zhang, X. K.,Liu, L.,He, Z. H.,Sun, D. J.,He, X. Y.,Xu, Z. H.,Zhang, P. P.,Chen, F.,Xia, X. C.,Zhang, X. K.,He, Z. H.. 2008

[3]Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21. Caixia Lan,Xiaowen Ni,Jun Yan,Yong Zhang,Xianchun Xia,Xinmin Chen,Zhonghu He.

[4]Diversity, distribution of Puroindoline genes and their effect on kernel hardness in a diverse panel of Chinese wheat germplasm. Ma, Xiaoling,Sajjad, Muhammad,Wang, Jing,Yang, Wenlong,Sun, Jiazhu,Li, Xin,Zhang, Aimin,Liu, Dongcheng,Ma, Xiaoling,Sajjad, Muhammad,Wang, Jing. 2017

[5]Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA. Hu, Ming-Jian,Zhang, Hai-Ping,Liu, Kai,Cao, Jia-Jia,Wang, Sheng-Xing,Jiang, Hao,Wu, Zeng-Yun,Lu, Jie,Zhu, Xiao F.,Xia, Xian-Chun,Sun, Gen-Lou,Ma, Chuan-Xi,Chang, Cheng,Xia, Xian-Chun,Sun, Gen-Lou. 2016

[6]Genome-wide identification and evolutionary analyses of bZIP transcription factors in wheat and its relatives and expression profiles of anther development related TabZIP genes. Li, Xueyin,Gao, Shiqing,Tang, Yimiao,Zhang, Fengjie,Feng, Biane,Fang, Zhaofeng,Zhao, Changping,Li, Xueyin,Ma, Lingjian,Li, Lei,Zhang, Fengjie,Feng, Biane. 2015

[7]Haplotype, molecular marker and phenotype effects associated with mineral nutrient and grain size traits of TaGS1 a in wheat. Guo, Ying,Sun, Jinjie,Zhang, Guizhi,Wang, Yingying,Kong, Fanmei,Zhao, Yan,Li, Sishen,Guo, Ying,Wang, Yingying. 2013

[8]Mineral element distributions in milling fractions of Chinese wheats. Tang, Jianwei,He, Zhonghu,Zhang, Yong,Tang, Jianwei,Qu, Yanying,Zou, Chunqin,Shi, Rongli,He, Zhonghu,Ortiz-Monasterio, Ivan. 2008

[9]Characterization of genome and chromosomes in octoploid wheat-wheatgrass amphiploid Zhong 2 using fluorescence in situ hybridization and chromosome pairing analysis. Gao, Z,Han, FP,He, MY,Ma, YZ,Xin, ZY. 1999

[10]Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Zhang, Hai,Li, Xing,Li, Zaifeng,Liu, Daqun,Xia, Xianchun,He, Zhonghu,He, Zhonghu. 2011

[11]Cytological and molecular analysis of wheat - Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat. Song, Liqiang,Lu, Yuqing,Zhang, Jinpeng,Pan, Cuili,Yang, Xinming,Li, Xiuquan,Liu, Weihua,Li, Lihui.

[12]The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Lillemo, M.,Asalf, B.,Bjornstad, A.,Singh, R. P.,Huerta-Espino, J.,Chen, X. M.,He, Z. H..

[13]Potassium (K) effects and QTL mapping for K efficiency traits at seedling and adult stages in wheat. Kong, Fan-Mei,Guo, Ying,Liang, Xue,Wu, Chun-Hong,Wang, Ying-Ying,Zhao, Yan,Li, Si-Shen,Guo, Ying,Wang, Ying-Ying.

[14]QTL mapping of grain arabinoxylan contents in common wheat using a recombinant inbred line population. Li Yang,Dehui Zhao,Jun Yan,Yelun Zhang,Xianchun Xia,Yubing Tian,Zhonghu He,Yong Zhang.

[15]Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat. Zhang, Zhi,Han, Haiming,Zhou, Shenghui,Zhang, Jinpeng,Yang, Xinming,Li, Xiuquan,Liu, Weihua,Li, Lihui,Song, Liqiang. 2017

[16]The impact of modern plant breeding on dominant Chinese wheat cultivars (Triticum aestivum L.) revealed by SSR and functional markers. Meng, Lingzhi,Xiang, Chao,Liu, Hongwei,Yang, Li,Li, Hongjie,Zhang, Hongjun,Zhou, Yang,Mai, Chunyan,Wei, Yunliang,Yu, Liqiang. 2018

[17]Determination of phenolic acid concentrations in wheat flours produced at different extraction rates. Wang, Lan,Yao, Yang,He, Zhonghu,Wang, Desen,Zhang, Yong,He, Zhonghu,Liu, Aihua. 2013

[18]QTL mapping of adult-plant resistance to stripe rust in a "Lumai 21xJingshuang 16' wheat population. He, Zhong H.,Xia, Xian C.,Ren, Yan,Liu, Li S.,He, Zhong H.,Wu, Ling,Bai, Bin.

[19]Molecular markers of Ph1 gene in Chinese spring and development of winter wheat new line harboring ph1b gene. Wang, XW,Lia, JR,Chen, LH,Liu, GT. 2000

[20]Phenolic acid profiles of Chinese wheat cultivars. He, Zhonghu,Zhang, Yong,Wang, Lan,Yao, Yang,Yan, Jun,He, Zhonghu,Yan, Jun.

作者其他论文 更多>>

微信小程序