Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis

文献类型： 外文期刊

第一作者： Zhang, Hua

作者： Zhang, Hua;Zou, Pan;Zhao, Haitian;Yang, Xin;Zou, Pan;Qiu, Junqiang;Mac Regenstein, Joe

作者机构：

关键词： Pinecones; Pinus koraiensis; PKP-E; Polysaccharide; Structure characterization

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN： 0144-8617

年卷期： 2021 年 251 卷

页码：

收录情况： SCI

摘要： The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of L-rhamnose, L-arabinose, D-mannose, D-glucose, and D-galactose. The fractions were mainly linked by 1 -> 6 or 1 -> glycosidic bonds and the backbone of 4 fractions was probably composed of -> 2, 6)-beta-D Man (1 -> and alpha-D-GalpA-(1 ->) which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS(center dot+) radicals were 3.0 and 23.6 mg/mL.

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