文献类型： 外文期刊
第一作者： Kong, Guanghui
作者： Kong, Guanghui;Li, Tinglu;Huang, Weixiong;Li, Minhui;Jiang, Liqun;Jiang, Zide;Xi, Pinggen;Shen, Wankuan;Hsiang, Tom;Jiang, Liqun;Xi, Pinggen
作者机构：
关键词： Peronophythora litchii; Lychee; Loop-mediated isothermal amplification; Nested PCR
期刊名称：CROP PROTECTION （ 影响因子：2.571； 五年影响因子：3.11 ）
ISSN： 0261-2194
年卷期： 2021 年 139 卷
页码：
收录情况： SCI
摘要： Downy blight caused by Peronophythora litchii is one of the most destructive diseases of lychee (Litchi chinensis Sonn.). Early diagnosis of the P. litchii pathogen should be the top priority for addressing disease epidemics and management. In this study, loop-mediated isothermal amplification (LAMP) assays targeting the M90 gene sequences were developed for specific detection of P. litchii. The detection limit of LAMP assay was 10 pg genomic DNA of P. litchii in 25 mu L reaction mixtures. LAMP assay and nested polymerase chain reaction (PCR) assay showed higher sensitivity than conventional PCR. The LAMP assay could also detect the presence of P. litchii in infected leaves, fruits and soils. This study provides a simple, highly sensitive, rapid and reliable protocol for the early diagnosis of P. litchii on lychee.
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