MdCAX affects the development of the 'Honeycrisp' bitter pit by influencing abnormal Ca distribution

文献类型： 外文期刊

第一作者： Liu, Jia

作者： Liu, Jia;Jiang, Zitao;Liu, Yanfei;Ding, Yuduan;Tian, Xiaoning;Ren, Xiaolin;Qi, Yingwei

作者机构：

关键词： Malus domestica; Bitter pit; CAX; Calcium distribution; 'Honeycrisp'

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：5.537； 五年影响因子：5.821 ）

ISSN： 0925-5214

年卷期： 2021 年 171 卷

页码：

收录情况： SCI

摘要： Bitter pit (BP) is a physiological disorder of calcium deficiency in apple fruit and has not been fully characterized. The Ca2+/H+ exchanger (CAX) is a kind of Ca2+ transport protein. In this study, we analysed mineral contents in different parts of fruit, the expression levels of genes related to Ca transport and the functional roles of MdCAXs. The results showed lower water-soluble Ca content and significantly higher ratios of water-soluble P/Ca and (K + Mg)/Ca in the calyx-end flesh of BP and normal fruit than the peduncle-end flesh of them. The expression level of MdCAX11 increased as the severity of BP increased, and the expression profile was negatively correlated with the water-soluble Ca content. Yeast growth assays showed that both MdCAX11 and MdCAX5 have the function of transporting Ca to vacuoles. Subcellular localization analysis showed that MdCAX11-GFP and MdCAX5-GFP were colocalized on the vacuole membrane with a tonoplast marker. Taken together, the results of this study indicate that high expression levels of MdCAX11 and MdCAX5 may cause an influx of Ca from the cytosol into vacuoles, which may be related to the occurrence of BP.

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