The UTP-glucose-1-phosphate uridylyltransferase of Brucella melitensis inhibits the activation of NF-kappa B via regulating the bacterial type IV secretion system

文献类型： 外文期刊

第一作者： Zhou, Yucheng

作者： Zhou, Yucheng;Chen, Yuening;Qiao, Lianjiang;Yang, Sen;Chen, Shipeng;Yang, Yanling;Ren, Linzhu;Bu, Zhaoyang;Wang, Xinglong;Qian, Jing;Yang, Yanling

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关键词： Brucella melitensis; UTP-glucose-1-phosphate uridylyltransferase (UGPase); Nuclear factor-kappa enhancer-binding protein (NF-kappa B); Type IV secretion system (T4SS)

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 164 卷

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收录情况： SCI

摘要： UDP-glucose pyrophosphorylase (UGPase) is an important pyrophosphatase that reversibly catalyzes the synthesis of UDP-glucose during glucose metabolism. We previously found that the deletion of UGPase may affect structure, growth, the virulence of Brucella, and the activation of cellular NF-kappa B. However, the exact mechanism of activation of NF-kappa B regulated by Brucella UGPase is still unclear. Here, we found for the first time that UGPase can regulate the expression of virB proteins (virB3, virB4, virB5, virB6, virB8, virB9, virB10, and virB11) of type IV secretion system (T4SS) as well as effectors (vceC, btpA, btpB, ricA, bspB, bspC, and bspF) of Brucella by promoting the expression of ribosomal S12 protein (rpsL), BMEI1825, and quinone of 2,4,5-trihydroxyphenylalanine (topA) proteins, and further inhibits the activation of cellular NF-kappa B and affects the virulence of Brucella. Our findings provide new insights into the mechanism used by Brucella to escape the immune recognition, which is expected to be of great value in the designing of Brucella vaccines and the screening of drug targets. (C) 2020 Elsevier B.V. All rights reserved.

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