RGG1, Involved in the Cytokinin Regulatory Pathway, Controls Grain Size in Rice

文献类型： 外文期刊

第一作者： Tao, Yajun

作者： Tao, Yajun;Wang, Jun;Li, Wenqi;Xu, Yang;Wang, Fangquan;Jiang, Yanjie;Chen, Zhihui;Fan, Fangjun;Yang, Jie;Tao, Yajun;Miao, Jun;Wang, Jun;Li, Wenqi;Xu, Yang;Wang, Fangquan;Jiang, Yanjie;Chen, Zhihui;Fan, Fangjun;Xu, Mengbin;Zhou, Yong;Liang, Guohua;Yang, Jie;Miao, Jun;Xu, Mengbin;Zhou, Yong;Liang, Guohua

作者机构：

关键词： Heterotrimeric G protein; RGG1; Rice; Grain size; Cytokinin

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Heterotrimeric GTP binding proteins (G proteins) and cytokinin play important roles in regulating plant growth and development. However, little is known about the mechanism by which they coordinate the regulation of grain size in rice. We functionally characterized one gene, RGG1, encoding a type-A G gamma subunit. Strong GUS staining was detected in young panicles and spikelets, suggesting a role for this gene in modulating panicle-related trait development. Overexpression of RGG1 in Nipponbare (NIP) and Wuyunjing 30 (WYJ30) significantly decreased plant height, panicle length and grain length by regulating cell division. However, rgg1 mutants generated by the CRISPR/Cas9 system exhibited no obvious phenotypic differences, which may be due to the extremely low expression level of this gene in vivo. The transcriptomes of young panicles of NIP, the NIP-rgg1-2 mutant and the NIP-OE2 overexpression line were sequenced, and the results showed that many differentially expressed genes (DEGs) were associated with the cytokinin biosynthetic pathway. We confirmed this result by measuring the endogenous cytokinin levels and found that cytokinin content was lower in the overexpression lines. Additionally, increased expression of RGG1 decreased sensitivity to low concentrations of 6-benzylaminopurine (6-BA). Our results reveal a novel G protein-cytokinin module controlling grain size in rice and will be beneficial for understanding the mechanisms by which G proteins regulate grain size and plant development.

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