A QuEChERS-HPLC-MS/MS Method with Matrix Matching Calibration Strategy for Determination of Imidacloprid and Its Metabolites in Procambarus clarkii (Crayfish) Tissues

文献类型： 外文期刊

第一作者： Yang, Qiuhong

作者： Yang, Qiuhong;Ai, Xiaohui;Dong, Jing;Liu, Yongtao;Zhou, Shun;Yang, Yibin;Xu, Ning;Ai, Xiaohui;Liu, Yongtao

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关键词： QuEChERS; HPLC-MS; MS; Imidacloprid; metabolites; 5-hydroxy imidacloprid; olefin imidacloprid; Imidacloprid urea; 6-chloronicotinic acid; Procambarus clarkii

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

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年卷期： 2021 年 26 卷 2 期

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收录情况： SCI

摘要： We developed a method for determination of imidacloprid and its metabolites 5-hydroxy imidacloprid, olefin imidacloprid, imidacloprid urea and 6-chloronicotinic acid in Procambarus clarkii (crayfish) tissues using quick, easy, cheap, effective, rugged, and safe (QuEChERS) and high-performance liquid chromatography-triple quadrupole mass spectrometry. Samples (plasma, cephalothorax, hepatopancrea, gill, intestine, and muscle) were extracted with acetonitrile containing 0.1% acetic acid and cleaned up using a neutral alumina column containing a primary secondary amine. The prepared samples were separated using reverse phase chromatography and scanned in the positive and negative ion multiple reaction-monitoring modes. Under the optimum experimental conditions, spiked recoveries for these compounds in P. clarkii samples ranged from 80.6 to 112.7% with relative standard deviations of 4.2 to 12.6%. The limits of detection were 0.02-0.5 mu g center dot L-1, the limits of quantification were 0.05-2.0 mu g center dot L-1 and the method of quantification was 0.05-2.0 mu g center dot kg(-1). The method is rapid, simple, sensitive and suitable for rapid determination and analysis of imidacloprid and its metabolites in P. clarkii tissues.

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