Non-specific DNAzyme-based biosensor with interfering ions for the Cd2+ determination in feed

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Zheng, Shanshan;Nan, Xuemei;Zhao, Yiguang;Wang, Yue;Zhang, Fan;Yang, Liang;Xiong, Benhai;Xu, Lixing;Wang, Yue

作者机构：

关键词： Non-specific DNAzyme; Field-effect transistor; Single-walled; carbon nanotube; heavy metal; Mathematical model; Feed

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN：

年卷期： 2021 年 329 卷

页码：

收录情况： SCI

摘要： Only parts of heavy metal ions have sensitive and selective deoxyribozymes(DNAzymes), but most of heavy metal ions do not have the specific DNAzyme that limits the development of high-precise biosensors. As for cadmium ion (Cd2+), it lacks a specific DNAzyme that can be interfered by the multiple heavy metal ions (Hg2+ and Pb2+), causing poor accuracy if only non-specific DNAzyme-based biosensor measures the Cd2+ concentration. Here, one approach to solve this problem is proposed that uses in conjunction with other biosensors and the analysis data model to explore the interference law and improve the detection accuracy. The non-specific DNAzyme-based biosensor is fabricated using a field-effect transistor(FET), single-walled carbon nanotube (SWNTs) and non-specific DNAzyme. The performances are characterized by different spectral and electronic methods, indicating excellent sensitivity, stability and repeatability. Under the optimized conditions, the nonspecific DNAzyme-based biosensor can determine Cd2+ down to 3.4 x 10(-2) nM, and the linear range is varying from 5.0 x 10(-2) to 1.0 x 10(3) nM with the correlation coefficient 0.98. To qualitatively and quantitatively analyze Cd2+ in a real sample, other biosensors with high sensitivity for Hg2+ and Pb2+ are selected and optimized to form a biosensor array, and then the detecting signals are processed by a mathematical model. At last, the prepared biosensor array is applied to measure the Cd2+ in feed to evaluate the detection accuracy.

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