Identification of long noncoding RNAs in silkworm larvae infected with Bombyx mori cypovirus

文献类型： 外文期刊

第一作者： Zhang, Zhendong

作者： Zhang, Zhendong;Zhao, Ze;Lin, Su;Wu, Wanming;Tang, Weiming;Dong, Youfu;Shen, Manman;Wu, Ping;Guo, Xijie;Zhang, Zhendong;Zhao, Ze;Lin, Su;Wu, Wanming;Tang, Weiming;Dong, Youfu;Shen, Manman;Wu, Ping;Guo, Xijie

作者机构：

关键词： Bombyx mori; cypovirus; DElncRNA– DEmRNA network; lncRNA; transcriptome analysis

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2021 年 106 卷 3 期

页码：

收录情况： SCI

摘要： Bombyx mori cypovirus (BmCPV) is one of the most important pathogens causing severe disease to silkworm. Emerging evidence indicates that long noncoding RNAs (lncRNAs) play importantly regulatory roles in virus infection and host immune response. To better understand the interaction between silkworm, Bombyx mori and BmCPV, we performed a comparative transcriptome analysis on lncRNAs and mRNAs between the virus-infected and noninfected silkworm larvae midgut at two time points postinoculation. A total of 16,753 genes and 1845 candidate lncRNAs were identified, among which 356 messenger RNA (mRNAs) and 41 lncRNAs were differentially expressed (DE). Target gene prediction revealed that most of DEmRNAs (123) were coexpressed with 28 DElncRNAs, suggesting that the expression of mRNA is mainly affected through trans- regulation by BmCPV-induced lncRNAs, and a regulatory network of DElncRNAs and DEmRNAs was then constructed. According to the network, many genes involved in apoptosis, autophagy, and antiviral response, such as ATG3, PDCD6, IBP2, and MFB1, could be targeted by different DElncRNAs, implying the essential roles of these genes and lncRNAs in BmCPV infection. In all, our studies revealed for the first time the alteration of lncRNA expression in BmCPV-infected larvae and its potential influence on BmCPV replication, providing a new perspective for host-cypovirus interaction studies.

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