A single nucleotide polymorphism variant located in the cis-regulatory region of the ABCG2 gene is associated with mallard egg colour

文献类型： 外文期刊

第一作者： Liu, Hehe

作者： Liu, Hehe;Hu, Jian;Guo, Zhanbao;Fan, Wenlei;Xu, Yaxi;Liang, Suyun;Liu, Dapeng;Zhang, Yunsheng;Xie, Ming;Tang, Jing;Huang, Wei;Zhang, Qi;Jiang, Yong;Feng, Yulong;Wu, Yongbao;Cao, Junting;Zhou, Zhengkui;Hou, Shuisheng;Liu, Hehe;Xi, Yang;Li, Yanying;Wang, Lei;Ma, Shengchao

作者机构：

关键词： ABCG2; causative mutation; eggshell colour; mallard

期刊名称：MOLECULAR ECOLOGY （ 影响因子：6.185； 五年影响因子：6.801 ）

ISSN： 0962-1083

年卷期： 2021 年 30 卷 6 期

页码：

收录情况： SCI

摘要： Avian egg coloration is shaped by natural selection, but its genetic basis remains unclear. Here, we used genome-wide association analysis and identity by descent to finely map green egg colour to a 179-kb region of Chr4 based on the resequencing of 352 ducks (Anas platyrhynchos) from a segregating population resulting from the mating of Pekin ducks (white-shelled eggs) and mallards (green-shelled eggs). We further narrowed the candidate region to a 30-kb interval by comparing genome divergence in seven indigenous duck populations. Among the genes located in the finely mapped region, only one transcript of the ABCG2 gene (XM_013093252.2) exhibited higher uterine expression in green-shelled individuals than in white-shelled individuals, as supported by transcriptome data from four populations. ABCG2 has been reported to encode a protein that functions as a membrane transporter for biliverdin. Sanger sequencing of the whole 30-kb candidate region (Chr4: 47.41-47.44 Mb) and a plasmid reporter assay helped to identify a single nucleotide polymorphism (Chr4: 47,418,074 G>A) located in a conserved predicted promoter region whose variation may alter ABCG2 transcription activity. We provide a useful molecular marker for duck breeding and contribute data to the research on ecological evolution based on egg colour patterns among birds.

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