Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin

文献类型： 外文期刊

第一作者： Li, Zhenhui

作者： Li, Zhenhui;Zheng, Ming;Mo, Jiawei;Li, Kan;Yang, Xin;Guo, Lijin;Zhang, Xiquan;Abdalla, Bahareldin Ali;Nie, Qinghua;Li, Zhenhui;Li, Zhenhui;Zheng, Ming;Mo, Jiawei;Li, Kan;Yang, Xin;Guo, Lijin;Zhang, Xiquan;Abdalla, Bahareldin Ali;Nie, Qinghua;Li, Zhenhui;Zheng, Ming;Mo, Jiawei;Li, Kan;Yang, Xin;Guo, Lijin;Zhang, Xiquan;Abdalla, Bahareldin Ali;Nie, Qinghua

作者机构：

关键词： ATGL; cell heterogeneity; melatonin; preadipocyte; single‐ cell RNA sequencing

期刊名称：JOURNAL OF PINEAL RESEARCH （ 影响因子：13.007； 五年影响因子：12.936 ）

ISSN： 0742-3098

年卷期： 2021 年 70 卷 3 期

页码：

收录情况： SCI

摘要： Obesity is a global epidemic health disorder and associated with several diseases. Body weight-reducing effects of melatonin have been reported; however, no investigation toward examining whether the beneficial effects of melatonin are associated with preadipocyte heterogeneity has been reported. In this study, we profiled 25 071 transcriptomes of normal and melatonin-treated preadipocytes using scRNA-seq. By tSNE analysis, we present a cellular-state landscape for melatonin-treated preadipocytes that covers multiple-cell subpopulations, defined as cluster 0 to cluster 13. Cluster 0 and cluster 1 were the largest components of normal and melatonin-treated preadipocytes, respectively. G0S2, an inhibitor of adipose triglyceride lipase (ATGL), was significantly upregulated in cluster 0 and downregulated in cluster 1. We redefined cluster 0 as the G0S2-positive cluster (G0S2(+)) and cluster 1 as the G0S2-negative cluster (G0S2(-)). Through pseudotime analysis, the G0S2(-) cluster cell differentiation trajectory was divided into three major structures, that is, the prebranch, the lipid catabolism branch, and the cell fate 2 branch. In vitro, G0S2 knockdown enhanced the expression levels of ATGL, BAT markers and fatty acid oxidation-related genes, but inhibited C/EBP alpha and PPAR gamma expression. In vivo, knockdown of G0S2 reduced the body weight gain in high-fat-fed mice. The beneficial effects of the G0S2(-) cell cluster in promoting lipolysis and inhibiting adipogenesis are dependent on two major aspects: first, downregulation of the G0S2 gene in the G0S2(-) cluster, resulting in activation of ATGL, which is responsible for the bulk of triacylglycerol hydrolase activity; and second, upregulation of FABP4 in the G0S2(-) cluster, resulting in inhibition of PPAR gamma and further reducing adipogenesis.

分类号：

  • 相关文献
作者其他论文 更多>>
  • The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics

    作者：Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Luo, Wen;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua

    关键词：TMEM182; Fat formation; Metabolomics; Lipidomics

  • The TaSnRK1-TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat

    作者：Lyu, Jinyang;Sun, Na;Yang, Fan;Li, Xuepeng;Mu, Junyi;Zhou, Runxiang;Zheng, Guolan;Yang, Xin;Zhang, Chenxuan;Han, Chao;Xia, Guang-Min;Fan, Min;Bai, Ming-Yi;Wang, Dongzhi;Xiao, Jun;Li, Genying;Xiao, Jun;Xiao, Jun

    关键词：wheat grain length; brassinosteroid; sugar; TabHLH489; SnRK1

  • GPCR-MAPK signaling pathways underpin fitness trade- offs in whitefly

    作者：Fu, Buli;Liang, Jinjin;Hu, Jinyu;Du, Tianhua;He, Chao;Wei, Xuegao;Gong, Peipan;Yang, Jing;Liu, Shaonan;Huang, Mingjiao;Yang, Xin;Zhang, Youjun;Fu, Buli;Hu, Jinyu;Liu, Kui;Du, Tianhua;Wei, Xuegao;Gui, Lianyou;Zhang, Youjun;Tan, Qimei;He, Chao;Liu, Shaonan;Huang, Mingjiao;Zhang, Youjun;Wei, Xuegao;Nauen, Ralf;Bass, Chris

    关键词：adaptive evolution; fitness trade- offs; GPCR; MAPK; P450

  • Dual mutations in the whitefly nicotinic acetylcholine receptor β1 subunit confer target-site resistance to multiple neonicotinoid insecticides

    作者：Yin, Cheng;Liu, Shao-Nan;Du, Tian-Hua;Gong, Pei-Pan;Wei, Xue-Gao;Yang, Jing;Huang, Ming-Jiao;Fu, Bu-Li;Liang, Jin-Jin;Xue, Hu;Hu, Jin-Yu;Ji, Yao;He, Chao;Du, He;Wang, Chao;Zhang, Rong;Lu, Han-Tang;Xie, Wen;Yang, Xin;Zhang, You-Jun;Yin, Cheng;Gui, Lian-You;OReilly, Andrias O.;Zhang, Cheng-Jia;Tan, Qi-Mei;Chu, Dong;Zhou, Xu-Guo;Nauen, Ralf;Bass, Chris

    关键词：

  • Using multiomics to explore the weight differences between genders in Muscovy ducks

    作者：Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Zhu, Weijian;Ji, Congliang

    关键词：Muscovy duck; sexual dimorphism; growth and development; RNA-seq; 16S rRNA sequencing technology

  • PMAIP1 promotes J subgroup avian leukosis virus replication by regulating mitochondrial function

    作者：Zhao, Yongxia;Zhao, Changbin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Zhao, Yongxia;Zhao, Changbin;Deng, Yuelin;Pan, Ming;Mo, Guodong;Liao, Zhiying;Zhang, Xiquan;Zhang, Dexiang;Li, Hongmei;Deng, Yuelin

    关键词：ALV-J; PMAIP1; mitochondrial function; immune; DF-1

  • Live chicken body fat measurement technology based on bio-electrical impedance

    作者：Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Deng, Yuelin

    关键词：BIA; Electrical impedance; Fat content; Broilers

微信小程序