文献类型： 外文期刊
第一作者： Wang, Xin
作者： Wang, Xin;Cheng, Yiluo;Zhang, Wenting;Lu, Qin;Wen, Guoyuan;Luo, Qingping;Shao, Huabin;Zhang, Tengfei;Wang, Xin;Pan, Zishu;Zhang, Wenting;Lu, Qin;Wen, Guoyuan;Luo, Qingping;Shao, Huabin;Zhang, Tengfei
作者机构：
关键词： Salmonella Pullorum; (p)ppGpp synthetases; Chicken; Pathogenicity
期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）
ISSN： 0944-5013
年卷期： 2021 年 245 卷
页码：
收录情况： SCI
摘要： Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important adaptation response induced by (p)ppGpp, and in Salmonella, (p)ppGpp is synthesized by two (p)ppGpp synthetases, RelA and SpoT. To investigate the role of (p)ppGpp synthetases in the adaptation and pathogenicity of S. Pullorum, a (p)ppGpp synthetases mutant (ArelAAspoT) was constructed, and its physiological phenotypes and pathogenicity, as well as transcription profiling, were compared with the parent strain. The ArelAAspoT mutant showed decreased ability to form biofilms, and reduced resistance to acidic, alkaline, high osmolarity and H2O2 conditions. The internalization of the ArelAAspoT mutant into host cells in vitro and its lethality and colonization abilities within young chickens were also significantly reduced. RNA sequencing showed that the (p)ppGpp synthetases did not only affect the classic stringent response, such as inhibition of DNA replication and protein synthesis, but also controlled the expression of many virulence factors, in particular, the Salmonella pathogenicity island 1 (SPI-1) and SPI-2 type III secretion systems (T3SSs), and adhesion factors. These results suggest that the (p)ppGpp synthetases are required for the pathogenicity of S. Pullorum by affecting its stress response and the expression of the virulence factors.
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