(p)ppGpp synthetases are required for the pathogenicity of Salmonella Pullorum in chickens
文献类型: 外文期刊
第一作者: Wang, Xin
作者: Wang, Xin;Cheng, Yiluo;Zhang, Wenting;Lu, Qin;Wen, Guoyuan;Luo, Qingping;Shao, Huabin;Zhang, Tengfei;Wang, Xin;Pan, Zishu;Zhang, Wenting;Lu, Qin;Wen, Guoyuan;Luo, Qingping;Shao, Huabin;Zhang, Tengfei
作者机构:
关键词: Salmonella Pullorum; (p)ppGpp synthetases; Chicken; Pathogenicity
期刊名称:MICROBIOLOGICAL RESEARCH ( 影响因子:5.415; 五年影响因子:6.038 )
ISSN: 0944-5013
年卷期: 2021 年 245 卷
页码:
收录情况: SCI
摘要: Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important adaptation response induced by (p)ppGpp, and in Salmonella, (p)ppGpp is synthesized by two (p)ppGpp synthetases, RelA and SpoT. To investigate the role of (p)ppGpp synthetases in the adaptation and pathogenicity of S. Pullorum, a (p)ppGpp synthetases mutant (ArelAAspoT) was constructed, and its physiological phenotypes and pathogenicity, as well as transcription profiling, were compared with the parent strain. The ArelAAspoT mutant showed decreased ability to form biofilms, and reduced resistance to acidic, alkaline, high osmolarity and H2O2 conditions. The internalization of the ArelAAspoT mutant into host cells in vitro and its lethality and colonization abilities within young chickens were also significantly reduced. RNA sequencing showed that the (p)ppGpp synthetases did not only affect the classic stringent response, such as inhibition of DNA replication and protein synthesis, but also controlled the expression of many virulence factors, in particular, the Salmonella pathogenicity island 1 (SPI-1) and SPI-2 type III secretion systems (T3SSs), and adhesion factors. These results suggest that the (p)ppGpp synthetases are required for the pathogenicity of S. Pullorum by affecting its stress response and the expression of the virulence factors.
分类号:
- 相关文献
作者其他论文 更多>>
-
Characterization of Two Novel Single-Stranded RNA Viruses from Agroathelia rolfsii, the Causal Agent of Peanut Stem Rot
作者:Yu, Dongyang;Wang, Qianqian;Song, Wanduo;Kang, Yanping;Lei, Yong;Wang, Zhihui;Chen, Yuning;Huai, Dongxin;Wang, Xin;Liao, Boshou;Yan, Liying
关键词:mitovirus; Agroathelia rolfsii; characterization; ArMV1; ArMV2
-
Species diversity of fish at the Wuzhizhou Island, South China Sea, based on environmental DNA
作者:Wang, Chongzhao;Cao, Kun;Xing, Yingchun;Wang, Chongzhao;Cao, Kun;Wang, Xin;Xi, Rui;Jiang, Ting;Yang, Rui;Xing, Yingchun;Wang, Xin;Xi, Rui;Jiang, Ting;Ma, Zhenhua;Yang, Rui
关键词:marine ecosystem; occurrence of fish taxon; distribution; sequences of MOTUs; the South China Sea
-
Effects of boiling, centrifuging and drying methods on bioactive compounds in Antarctic krill meal
作者:Yang, Liu;Zhang, Shuaishuai;Wang, Xin;Yang, Liu;Zheng, Yao;Zhang, Shuaishuai;Guo, Quanyou;Yang, Liu;Zheng, Yao;Zhang, Shuaishuai;Guo, Quanyou
关键词:Antarctic krill; drying methods; manufacturing process; bioactive compounds
-
Soil Fungal Community Differences in Manual Plantation Larch Forest and Natural Larch Forest in Northeast China
作者:Wang, Mingyu;Sui, Xin;Wang, Xin;Wang, Mingyu;Sui, Xin;Wang, Xin;Wang, Mingyu;Sui, Xin;Wang, Xin;Wang, Mingyu;Sui, Xin;Wang, Xin;Zhang, Xianbang;Zeng, Xiannan
关键词:manual plantation larch forest; natural larch forest; soil fungal communities; co-occurrence network; community assembly
-
Identification of QTLs associated with very-long chain fatty acid (VLCFA) content via linkage mapping and BSA-seq in peanut
作者:Xue, Xiaomeng;Li, Jianguo;Wu, Jie;Hu, Meiling;Liu, Nian;Yan, Liying;Chen, Yuning;Wang, Xin;Kang, Yanping;Wang, Zhihui;Jiang, Huifang;Lei, Yong;Liao, Boshou;Huai, Dongxin;Xue, Xiaomeng;Zhang, Chunyu
关键词:
-
Heat stress and sexual reproduction in maize: unveiling the most pivotal factors and the greatest opportunities
作者:Lv, Xuanlong;Yao, Qian;Mao, Fen;Liu, Mayang;Wang, Yudong;Wang, Xin;Liao, Shuhua;Wang, Pu;Huang, Shoubing;Gao, Yingbo;Wang, Yuanyuan
关键词:Flowering stage; high temperature; pollen tube; seed set; sexual organs; Zea mays
-
Translatome and Transcriptome Analyses Reveal the Mechanism that Underlies the Enhancement of Salt Stress by the Small Peptide Ospep5 in Plants
作者:Wang, Jinyan;Li, Yang;Li, Mingyue;Zhang, Wenting;Ling, Xitie;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Wang, Jinyan;Li, Yang;Li, Mingyue;Zhang, Wenting;Ling, Xitie;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Wang, Jinyan;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Zheng, Zhongbing;Zhang, Baolong;Liu, Qing;Lu, Yaping;Hua, Kai
关键词:translatome; transcriptome; peptidome; small peptides; rice salt tolerance