Genome structure and diversity of novel endornaviruses from wheat sharp eyespot pathogen Rhizoctonia cerealis

文献类型： 外文期刊

第一作者： Li, Wei

作者： Li, Wei;Zhang, Haotian;Shu, Yan;Cao, Shulin;Sun, Haiyan;Zhang, Aixiang;Chen, Huaigu;Li, Wei;Chen, Huaigu;Zhang, Haotian

作者机构：

关键词： Endornavirus; Rhizoctonia cerealis; Diversity; Genome structure

期刊名称：VIRUS RESEARCH （ 影响因子：2.934； 五年影响因子：2.775 ）

ISSN： 0168-1702

年卷期： 2021 年 297 卷

页码：

收录情况： SCI

摘要： Rhizoctonia cerealis (teleomorph Ceratobasidium cereale) is a soil-borne plant pathogenic fungus that can cause sharp eyespot in wheat or yellow patch in grasses. In this study, 21 new endornavirus genomes were obtained from five R. cerealis strains through the high-throughput sequencing of viral double-stranded RNA. Eighteen viruses were identified as Alphaendornavirus, and three viruses were identified as new species of Betaendornavirus on the basis of the phylogenetic analysis of the deduced amino acid sequences of RNA-dependent RNA polymerase. Notably, 12 of the new alphaendornaviruses could encode two open reading frames (ORFs), which were a rare feature of Endornaviridae. The amino acid sequences encoded by ORF2 from different endornaviruses had very low identity, and their functions and evolution origins remained unclear. Different endornavirus species with remarkably different genome structures could be found in the same R. cerealis strain. This study indicated that endornaviruses are common in R. cerealis and display wide diversity. Betaendornaviruses were found in R. cerealis, and a new species was proposed. This study is the first to report that the endornaviruses from R. cerealis can encode two ORFs and enhances our understanding of the viruses in the Endornaviridae family.

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