Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung

文献类型： 外文期刊

第一作者： Li, Xiao

作者： Li, Xiao;Zhang, Lida;Huang, Fuyan;Zhao, Junwei;Wang, Han;Jiao, Yanjie;Qian, Lulu;Wang, Xiangjing;Xiang, Wensheng;Xiang, Wensheng

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关键词： Genome; Microbacterium helvum sp; nov; Polyphasic taxonomy; 16S rRNA gene

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：1.884； 五年影响因子：1.835 ）

ISSN： 0302-8933

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收录情况： SCI

摘要： A Gram-positive, aerobic, non-motile, non-spore-forming, short rod-shaped strain, NEAU-LLCT, was isolated from cow dung in Shangzhi City, Heilongjiang Province, Northeast China and identified by a polyphasic taxonomic study. Colonies was light yellow, round, with entire margin. Strain NEAU-LLCT was grown at 15-45 celcius and pH 6.0-10.0. NaCl concentration ranged from 0 to 5% (W/V). The 16S rRNA gene sequence of NEAU-LLCT showed the high similarities with Microbacterium kyungheense JCM 18735(T) (98.5%), Microbacterium trichothecenolyticum JCM 1358(T) (98.3%) and Microbacterium jejuense JCM 18734(T) (98.2%). The whole-cell sugars were glucose, rhamnose and ribose. The menaquinones contained MK-12 and MK-13. Ornithine, glutamic acid, lysine and a small amount of alanine and glycine were the amino acids in the hydrolyzed products of the cell wall. The major fatty acids were iso-C-16:0, iso-C-18:0, anteiso-C-15:0 and anteiso-C-17:0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The genome of NEAU-LLCT was 4,369,375 bp and G + C content is 70.28 mol%. A combination of DNA-DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-LLCT could be distinguished from its closely related strains. Therefore, the strain NEAU-LLCT was considered to represent a novel species, which was named Microbacterium helvum sp. (Type strain NEAU-LLCT = CCTCC AA 2018026(T) = JCM 32661(T)).

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