First Report on Natural Infection of Nodavirus in an Echinodermata, Sea Cucumber (Apostichopus japonicas)

文献类型： 外文期刊

第一作者： Wang, Chong

作者： Wang, Chong;Li, Chenghua;Zhang, Qingli;Wang, Chong;Yao, Liang;Wang, Wei;Sang, Songwen;Hao, Jingwei;Zhang, Qingli

作者机构：

关键词： covert mortality nodavirus (CMNV); natural infection; sea cucumber (Apostichopus japonicas); in situ hybridization; TEM

期刊名称：VIRUSES-BASEL （ 影响因子：3.816； 五年影响因子：4.001 ）

ISSN：

年卷期： 2021 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Cross-species transmission of emerging viruses happens occasionally due to epidemiological, biological, and ecological factors, and it has caused more concern recently. Covert mortality nodavirus (CMNV) was revealed to be a unique shrimp virus that could cross species barrier to infect vertebrate fish. In the present study, CMNV reverse transcription-nested PCR (RT-nPCR)-positive samples were identified from farmed sea cucumber (Apostichopus japonicas) in the CMNV host range investigation. The amplicons of RT-nPCR from sea cucumber were sequenced, and its sequences showed 100% identity with the RNA-dependent RNA polymerase gene of the original CMNV isolate. Histopathological analysis revealed pathologic changes, including karyopyknosis and vacuolation of the epithelial cells, in the sea cucumber intestinal tissue. The extensive positive hybridization signals with CMNV probe were shown in the damaged epithelial cells in the in situ hybridization assay. Meanwhile, transmission electron microscopy analysis revealed CMNV-like virus particles in the intestine epithelium. All the results indicated that the sea cucumber, an Echinodermata, is a new host of CMNV. This study supplied further evidence of the wide host range of CMNV and also reminded us to pay close attention to its potential risk to threaten different aquaculture animal species.

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