Determination of acetylgestagens in animal-derived matrix samples using enhanced matrix removal lipid clean-up in combination with ultra performance liquid chromatography-tandem mass spectrometry

文献类型： 外文期刊

第一作者： Zhang, Congcong

作者： Zhang, Congcong;Zhang, Qingyang;Yin, Zenghao;Chen, Gang;Zheng, Lufei;Hu, Jian;Ma, Aijin

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关键词： Acetylgestagens; Animal-derived matrix sample; EMR-lipid cartridge; Liquid chromatography-tandem mass spectrometry

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.049； 五年影响因子：3.861 ）

ISSN： 0021-9673

年卷期： 2021 年 1649 卷

页码：

收录情况： SCI

摘要： A robust and confirmative method was established for the determination of six acetylgestagen residues, namely, flurogestone acetate (FGA), megestrol (MA), melengestrol acetate (MGA), chlormadinone acetate (CMA), medroxyprogesterone (MPA), and hydroxyprogesterone acetate (HPA) in animal-derived matrix samples by utilizing enhanced matrix removal lipid (EMR-lipid) clean-up in combination with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The analytes were extracted with acetonitrile, purified with a EMR-lipid cartridge, and separated with a reversed-phase C18 column. The limit of quantification (S/N >= 10) for CMA, FGA, HPA, MA, and MGA in all matrices was 0.5 ng/g, and for MPA, it was 1.0 ng/g; the limit of detection (S/N >= 3) for CMA, FGA, HPA, MA, and MGA in all matrices was 0.1 ng/g, and for MPA, it was 0.2 ng/g. The recoveries were between 61.0% and 114.8%, and the relative standard deviations (RSDs) were below 12%. The method was calibrated in a matrix-assisted standard solution in various linear ranges for the analytes and matrices, and the correlation coefficients (R-2) exceeded 0.99 for all the matrices. (c) 2021 Elsevier B.V. All rights reserved.

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