Genome-Wide Identification and Expression Analysis of the Melon Aldehyde Dehydrogenase (ALDH) Gene Family in Response to Abiotic and Biotic Stresses

文献类型： 外文期刊

第一作者： Yang, Dekun

作者： Yang, Dekun;Chen, Hongli;Zhang, Yu;Wang, Yan;Zhai, Yongqi;Xu, Gang;Ding, Qiangqiang;Wang, Mingxia;Zhang, Qi-an;Yan, Congsheng;Yang, Dekun;Chen, Hongli;Zhang, Yu;Wang, Yan;Zhai, Yongqi;Xu, Gang;Ding, Qiangqiang;Wang, Mingxia;Zhang, Qi-an;Yan, Congsheng;Zhang, Yu;Lu, Xiaomin;Yang, Dekun;Chen, Hongli

作者机构：

关键词： ALDH gene family; melon; abiotic and biotic stress; RNA-seq

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 20 期

页码：

收录情况： SCI

摘要： Through the integration of genomic information, transcriptome sequencing data, and bioinformatics methods, we conducted a comprehensive identification of the ALDH gene family in melon. We explored the impact of this gene family on melon growth, development, and their expression patterns in various tissues and under different stress conditions. Our study discovered a total of 17 ALDH genes spread across chromosomes 1, 2, 3, 4, 5, 7, 8, 11, and 12 in the melon genome. Through a phylogenetic analysis, these genes were classified into 10 distinct subfamilies. Notably, genes within the same subfamily exhibited consistent gene structures and conserved motifs. Our study discovered a pair of fragmental duplications within the melon ALDH gene. Furthermore, there was a noticeable collinearity relationship between the melon's ALDH gene and that of Arabidopsis (12 times), and rice (3 times). Transcriptome data reanalysis revealed that some ALDH genes consistently expressed highly across all tissues and developmental stages, while others were tissue- or stage-specific. We analyzed the ALDH gene's expression patterns under six stress types, namely salt, cold, waterlogged, powdery mildew, Fusarium wilt, and gummy stem blight. The results showed differential expression of CmALDH2C4 and CmALDH11A3 under all stress conditions, signifying their crucial roles in melon growth and stress response. RT-qPCR (quantitative reverse transcription PCR) analysis further corroborated these findings. This study paves the way for future genetic improvements in melon molecular breeding.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序