Characterization of a Powdery Mildew Resistance Gene in Wheat Breeding Line Jingzi 102 Using Bulk Segregant RNA Sequencing

文献类型： 外文期刊

第一作者： Xing, Lixian

作者： Xing, Lixian;Gu, Tiantian;Shi, Fengyu;Jin, Yuli;Han, Guohao;Xu, Hongxing;An, Diaoguo;Fu, Xiaoyi;He, Mingqi;Zhou, Yilin;Liu, Wei;Xing, Lixian;Gu, Tiantian

作者机构：

关键词： bulked segregant RNA sequencing (BSR-Seq); marker-assisted selection; molecular mapping; powdery mildew; wheat breeding line

期刊名称：PLANT DISEASE （ 影响因子：4.4； 五年影响因子：4.8 ）

ISSN： 0191-2917

年卷期： 2024 年 108 卷 10 期

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收录情况： SCI

摘要： Wheat (Triticum aestivum L.) is one of the most important crops worldwide. Powdery mildew caused by Blumeria graminis f. sp. tritici is a destructive disease threatening wheat yield and quality. The utilization of resistant genes and cultivars is considered the most economical, environmentally friendly, and effective method to control powdery mildew. Wheat breeding line Jingzi 102 was highly resistant to powdery mildew at both seedling and adult plant stages. Genetic analysis of F1, F2, and F2:3 populations of "Jingzi 102 x Shixin 828" showed that the resistance of Jingzi 102 against powdery mildew isolate E09 at the seedling stage was controlled by a single dominant gene, temporarily designated PmJZ. Using bulked segregant RNA sequencing combined with molecular markers analysis, PmJZ was located on the long arm of chromosome 2B and flanked by markers BJK695-1 and CIT02g-20 with the genetic distances of 1.2 and 0.5 centimorgan, respectively, corresponding to the bread wheat genome of Chinese Spring (International Wheat Genome Sequencing Consortium RefSeq v2.1) 703.8 to 707.6 Mb. PmJZ is most likely different from the documented Pm genes on chromosome 2BL based on their physical positions, molecular markers analysis, and resistance spectrum. Based on the gene annotation information, five genes related to disease resistance could be considered as the candidate genes of PmJZ. To accelerate the application of PmJZ, the flanking markers BJK695-1 and CIT02g-20 can serve for marker-assisted selection of PmJZ in wheat disease-resistance breeding.

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