Exploration of the mechanism of a novel enhancer on buffalo muscle development

文献类型： 外文期刊

第一作者： Zhang, Feng

作者： Zhang, Feng;Xiong, Qi;Chen, Mengjie;Duan, Saixing;Xu, Gaoxiao;Duan, Saixing;Liu, Xingyu;Liu, Wenjing;Dou, Yuting;Geng, Kuan

作者机构：

关键词： Enhancer; Promoter; Muscle; Buffalo; Transcriptions

期刊名称：GENOMICS （ 影响因子：3.0； 五年影响因子：3.3 ）

ISSN： 0888-7543

年卷期： 2025 年 117 卷 4 期

页码：

收录情况： SCI

摘要： The developmental process of skeletal muscle involves a wide variety of regulatory factors. However, little has been reported regarding the function and mechanisms of enhancer regulation in buffalo skeletal muscle growth and development. In this study, we discovered an enhancer that affects the buffalo skeletal muscle growth and development, which we named Enh67. Experimental results from CCK-8, EdU, qPCR, and Western blot assays indicated that Enh67 inhibits the proliferation of buffalo muscle stem cells. Immunofluorescence staining, qPCR, and Western blot results showed that Enh67 promotes the differentiation of buffalo muscle stem cells. Finally, we determined through 3C and qPCR assays that Enh67 targets CEP85L. Our findings not only enrich the regulatory factors involved in buffalo skeletal muscle growth and development but also provide potential target candidates for improving meat production and treating muscle development-related diseases in livestock.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序