Identification of the microorganisms for methane-dependent arsenate reduction in wetland using DNA-stable isotope probing and metagenomics

文献类型： 外文期刊

第一作者： Yu, Xiaoxiao

作者： Yu, Xiaoxiao;Zhou, Yujie;Chen, Yun;Xu, Jianming;Tang, Xianjin;Li, Jibing;Luo, Chunling;Li, Jibing;Luo, Chunling;Zou, Lina;Shen, Chaofeng;Liu, Fengjie;Tang, Xianjin

作者机构：

关键词： Methane oxidation; Arsenate reduction; DNA-SIP; Metagenomic; Wetland

期刊名称：WATER RESEARCH （ 影响因子：12.4； 五年影响因子：12.9 ）

ISSN： 0043-1354

年卷期： 2025 年 284 卷

页码：

收录情况： SCI

摘要： The methane-dependent arsenate reduction (M-AsR) process is recently demonstrated to enhance the release of mobile and toxic arsenite [As(III)], with critical implications for ecosystem safety in wetland ecosystem. However, the key functional microorganisms and the underlying metabolic mechanisms of M-AsR in wetland remain unclear. In this study, 13C-labeled methane (CH4) was used as the sole carbon source to track the active microorganisms responsible for M-AsR. DNA-stable isotope probing (DNA-SIP) combined with amplicon and metagenomic sequencing was further employed to identify the microorganisms involved in M-AsR. The results showed that arsenate [As(V)] reduction occurred exclusively in the treatment amended with both CH4 and As(V). After a 50-day incubation, significant shifts in the relative abundance of functional genes (pmoA, ANME-2d mcrA and arrA) were observed in the heavy DNA fractions from the treatment amended with 13CH4 and As(V), indicating the incorporation of 13C into M-AsR microorganisms. Furthermore, the Methanobacterium, Methylobacter and arsenate-reducing bacteria (Chryseobacterium and Hydrogenophaga) were the predominant genera in 13CH4As heavy fractions and identified as the potential microorganisms responsible for M-AsR in wetland. Metagenomic analysis further confirmed that most of these microorganisms contained genes for CH4 oxidation and As (V) reduction. This multi-omics approach provides mechanistic insights into microbial mediated As(V) reduction in methane-rich wetland area.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序