Construction and characterization of partially ntrC-deleted mutants in Alcaligenes faecalis

文献类型: 外文期刊

第一作者: Cheng, HM

作者: Cheng, HM;Lin, M;Ping, SZ;Jia, SR

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关键词: Alcaligenes faecalis;ntrC mutant;expression and regulation of nif genes

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

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收录情况: SCI

摘要: To study the effect of ntrC gene product on the expression andregulation of other important nitrogen-fixing genes in Alcaligenesfaecalis, partially ntrC-deleted mutants of A. faecalis have beengenerated. To start with, the ntrC gene of A. faecalis was clonedinto a suicide plasmid pSUP202 to create a recombinant plasmid pSUM1.The ntrC gene in pSUM1 was then replaced by a lacZ-Km fragmentresulted in the generation of a plasmid pSUM2.

分类号: N1

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