The SUMOylation pathway regulates the pathogenicity of Fusarium oxysporum f. sp. niveum in watermelon through stabilizing the pH regulator FonPalC via SUMOylation

文献类型： 外文期刊

第一作者： Azizullah

作者： Azizullah;Noman, Muhammad;Gao, Yizhou;Wang, Hui;Xiong, Xiaohui;Wang, Jiajing;Li, Dayong;Song, Fengming;Azizullah;Noman, Muhammad;Gao, Yizhou;Wang, Hui;Xiong, Xiaohui;Wang, Jiajing;Li, Dayong;Song, Fengming;Azizullah;Noman, Muhammad;Gao, Yizhou;Wang, Hui;Xiong, Xiaohui;Wang, Jiajing;Li, Dayong;Song, Fengming;Noman, Muhammad

作者机构：

关键词： FonSMT3; FonPalC; Fusarium oxysporum f. sp. niveum; Pathogenicity; SUMOylation; Watermelon Fusarium wilt

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.7； 五年影响因子：7.1 ）

ISSN： 0944-5013

年卷期： 2024 年 281 卷

页码：

收录情况： SCI

摘要： SUMOylation is a key post-translational modification, where small ubiquitin-related modifier (SUMO) proteins regulate crucial biological processes, including pathogenesis, in phytopathogenic fungi. Here, we investigated the function and mechanism of the SUMOylation pathway in the pathogenicity of Fusarium oxysporum f. sp. niveum (Fon), the fungal pathogen that causes watermelon Fusarium wilt. Disruption of key SUMOylation pathway genes, FonSMT3, FonAOS1, FonUBC9, and FonMMS21, significantly reduced pathogenicity, impaired penetration ability, and attenuated invasive growth capacity of Fon. Transcription and proteomic analyses identified a diverse set of SUMOylation-regulated differentially expressed genes and putative FonSMT3-targeted proteins, which are predicted to be involved in infection, DNA damage repair, programmed cell death, reproduction, growth, and development. Among 155 putative FonSMT3-targeted proteins, FonPalC, a Pal/Rim-pH signaling regulator, was confirmed to be SUMOylated. The FonPalC protein accumulation was significantly decreased in SUMOylation-deficient mutant Delta Fonsmt3. Deletion of FonPalC resulted in impaired mycelial growth, decreased pathogenicity, enhanced osmosensitivity, and increased intracellular vacuolation in Fon. Importantly, mutations in conserved SUMOylation sites of FonPalC failed to restore the defects in Delta Fonpalc mutant, indicating the critical function of the SUMOylation in FonPalC stability and Fon pathogenicity. Identifying key SUMOylation-regulated pathogenicity-related proteins provides novel insights into the molecular mechanisms underlying Fon pathogenesis regulated by SUMOylation.

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