Preparation and evaluation of virus-like particle vaccine against H3N8 subtype equine influenza

文献类型： 外文期刊

第一作者： Zhang, Pengju

作者： Zhang, Pengju;Tan, Chengcheng;Li, Xintao;Wang, Weixia;Sun, Yixue;Ling, Mengmeng;Cong, Yanlong

作者机构：

关键词： Equine influenza; Virus-like particles; Hyperimmune serum; Preventive and therapeutic efficacy

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.664 ）

ISSN： 0882-4010

年卷期： 2021 年 157 卷

页码：

收录情况： SCI

摘要： To prevent and control H3N8 subtype equine influenza, we prepared virus-like particles (VLPs) comprising the HA, NA and M1 proteins of H3N8 equine influenza virus (EIV) through the insect cell-baculovirus expression system. The results of Western blot and hemagglutination analyses demonstrated that the constructed VLPs comprising HA, NA and M1 proteins have good hemagglutination activity. Immunoelectron microscope revealed that the VLPs share similar morphology and structure with natural virus particles. The hyperimmune serum from horses immunized with the VLPs were injected into mice by means of artificial passive immunization and then challenge, or challenge following by injecting hyperimmune serum. The results showed that the equine hyperimmune serum has good preventive and therapeutic efficacy against the infection of H3N8 EIV. The study provides a technical foundation for the development of H3N8 EIV VLP vaccine.

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