Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis

文献类型： 外文期刊

第一作者： Xiuming Wu

作者： Xiuming Wu;Fuguang Li#2;;Chaojun Zhang;Chuanliang Liu;Xueyan Zhang

作者机构：

关键词： Gossypium hirsutum;cotton;callus culture;somatic embryogenesis;gene expression regulation;DNA libraries;complementary DNA;expressed sequence tags;sequence analysis

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Regeneration is an essential step for recovery of transgenic plants following gene transfer. However, most cotton cultivars fail to respond to the current regeneration protocols for cotton. This hinders the use of gene transfer technology to improve this crop. Identification of the genes involved in cotton somatic embryogenesis (SE) may provide information that will help to improve regeneration protocols. To investigate the genes expressed during cotton SE, we constructed a suppression subtractive hybridization (SSH) library using cDNA from nonembryogenic callus as driver and those from embryogenic callus as tester. From this library, 368 cDNA clones that hybridized conspicuously to the forward-subtracted and unsubtracted tester probes, but not to the reverse-subtracted or unsubtracted driver probes, were obtained and sequenced. Among the 241 putative unigenes, the functions of 152 genes (63%) could be assigned using existing databases. In addition to many previously reported SE-related genes, some new genes, such as members of ethylene pathway and auxin pathway, were discovered in our library. The expression of eight genes, including an Aux/IAA responsive gene, was analyzed by reverse-transcription-polymerase chain reaction and found to be upregulated during the SE. This is in agreement with previous studies showing that embryogenesis involves auxin signaling.

分类号： Q94

  • 相关文献

[1]Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza sativa L.. Qin, Qiu-lin,Liu, Jin-ge,Zhang, Zhen,Peng, Ri-he,Xiong, Ai-sheng,Yao, Quan-hong,Chen, Jian-min.

[2]Construction, Characterization, and Expressed Sequence Tag (EST) Analysis of Normalized cDNA Library of Thermo-Photoperiod-Sensitive Genic Male Sterile (TPGMS) Wheat from Spike Developmental Stages. Yang, D.,Tang, Z. H.,Zheng, Y. L.,Tang, Z. H.,Zhang, L. P.,Zhao, C. P..

[3]Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.). Zhenzhen Xu,Chaojun Zhang,Xiaoyang Ge,Ni Wang,Kehai Zhou,Xiaojie Yang,Zhixia Wu,Xueyan Zhang,Chuanliang Liu,Zuoren Yang,Changfeng Li,Kun Liu,Zhaoen Yang,Yuyuan Qian,Fuguang Li.

[4]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[5]Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24. Xu Zhenzhen. 2013

[6]QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Fu-Ding Sun,Jian-Hong Zhang,Shu-Fang Wang,Wan-Kui Gong,Yu-Zhen Shi,Ai-Ying Liu,Jun-Wen Li,Ju-Wu Gong,Hai-Hong Shang,You-Lu Yuan.

[7]Modelling the structural response of cotton plants to mepiquat chloride and population density. Gu, Shenghao,Zhang, Lizhen,Evers, Jochem B.,van der Werf, Wopke,Mao, Lili,Li, Zhaohu,Zhang, Siping,Zhao, Xinhua,Liu, Shaodong,Zhang, Lizhen,Li, Zhaohu. 2014

[8]Effect of auxin, cytokinin and nitrogen on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f.niedzwetzkyana). Ji, Xiao-Hao,Wang, Yan-Ting,Zhang, Rui,Wu, Shu-Jing,An, Meng-Meng,Li, Min,Chen, Xiao-Liu,Zhang, Yan-Min,Chen, Xue-Sen,Ji, Xiao-Hao,Wang, Yan-Ting,Zhang, Rui,Wu, Shu-Jing,An, Meng-Meng,Li, Min,Chen, Xiao-Liu,Zhang, Yan-Min,Chen, Xue-Sen,Ji, Xiao-Hao,Wang, Yan-Ting,Zhang, Rui,Wu, Shu-Jing,An, Meng-Meng,Li, Min,Chen, Xiao-Liu,Chen, Xue-Sen,Wang, Chuan-Zeng,Zhang, Yan-Min.

[9]Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice. Yin, Yan-hui,Li, Bi-chun,Wei, Guang-hui,Zhu, Cai-ye,Li, Wei,Zhang, Ya-ni,Du, Li-xin,Cao, Wen-guang.

[10]Molecular Characterization and Expression Pattern of Two General Odorant Binding Proteins from the Diamondback Moth, Plutella xylostella. Zhang, Zhi-Chun,Wang, Man-Qun,Zhang, Guoan,Lu, Yao-Bin.

[11]Differential Gene Expression Analysis of Yunnan Red Pear, Pyrus Pyrifolia, During Fruit Skin Coloration. Zhang, Xiaodong,Yi, Qiong,Chen, Limei,Li, Kunzhi,Shu, Qun,Su, Jun,Allan, Andrew C..

[12]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[13]Functional annotation of an expressed sequence tag library from Haliotis diversicolor and analysis of its plant-like sequences. Jiang, Jing-Zhe,Guo, Zhi-Xun,Su, You-Lu,Wang, Rui-Xuan,Wang, Jiang-Yong,Zhang, Wei,Cai, Chen-Chen.

[14]Manganese Regulates Manganese-Containing Superoxide Dismutase (MnSOD) Expression in the Primary Broiler Myocardial Cells. Gao, Tianquan,Zhang, Keying,Li, Sufen,Luo, Xugang,Li, Sufen,Luo, Xugang,Wang, Funing,Li, Sufen. 2011

[15]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

[16]A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhang, Qi,Lei, Cai-Lin,Zhang, Shi-Hong.

[17]Molecular identification, polymorphism, and expression analysis of major histocompatibility complex class IIA and B genes of turbot (Scophthalmus maximus). Zhang, Yu-Xi,Chen, Song-Lin.

[18]Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.). Chen, Ming,Xu, Zhaoshi,Xia, Lanqin,Li, Liancheng,Dong, Jianhui,Wang, Qiaoyan,Ma, Youzhi,Cheng, Xianguo.

[19]Physiological investigation of C-4-phosphoenolpyruvate-carboxylase-introduced rice line shows that sucrose metabolism is involved in the improved drought tolerance. Zhang, Chen,Li, Xia,He, Yafei,Zhang, Jinfei,Yan, Ting,Liu, Xiaolong,Zhang, Chen,Li, Xia,Yan, Ting.

[20]Structure and Regulation of the gab Gene Cluster, Involved in the gamma-Aminobutyric Acid Shunt, Are Controlled by a sigma(54) Factor in Bacillus thuringiensis. Song, Fuping,Jiang, Yanan,Sun, Changpo,Zhang, Jie,Zhu, Li,Sun, Changpo,Huang, Dafang,Peng, Qi.

作者其他论文 更多>>

微信小程序