Target of Rapamycin (TOR) Regulates the Expression of IncRNAs in Response to Abiotic Stresses in Cotton

文献类型： 外文期刊

第一作者： Yun Song;;Linxuan Li;;Zhaoen Yang;;Ge Zhao

作者： Yun Song;Linxuan Li;Zhaoen Yang;Ge Zhao;Xueyan Zhang;Lingling Wang;Lei Zheng;Fengping Zhuo;Huan Yin;Xiaoyang Ge;Chaojun Zhang;Zuoren Yang;Maozhi Ren;Fuguang Li

作者机构：

关键词： target of rapamycin; expression pattern; stress response; cotton; long non-coding RNA

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： TOR (Target of Rapamycin) kinase is an evolutionarily conserved protein kinase, which integrates stress-related cues with growth and metabolic outputs. Long non-coding RNAs (IncRNAs) play a vital role in the regulation of eukaryotic genes. However, little is known about TOR's function in regulating the expression of IncRNAs in plants. In this study, four putative homologous genes encoding the TOR protein were identified by utilizing the recently completed cotton genome. Pharmacological experiments with TOR inhibitor AZD8055 and on silencing GhTOR genes resulted in obvious cotton growth retardation, indicating the conserved role of TOR in plant growth. The expression pattern analyses in different tissues reveal that TOR may play a role in root development, and the transcript levels of TOR genes were changed under different stress conditions. Importantly, we found TOR may be a key player in regulating the expression of long non-coding RNAs (IncRNAs). A total of 10,315 IncRNAs were discovered in cotton seedlings, 90.7% of which were long intergenic ncRNAs. Moreover, we identified the differentially expressed IncRNAs, of which 296 were significantly upregulated and 105 were downregulated in TOR inactivated plants. GO and KEGG analyses of differentially expressed IncRNA neighboring genes reveal that these differentially expressed IncRNA-targeted genes are involved in many life processes, including stress response, glutathione, and ribosomes in cotton. A series of differentially expressed IncRNAs potentially involved in plant stress response was identified under TOR inhibition. Collectively, these results suggest that cotton TOR proteins may directly modulate the expression of putative stress-related IncRNAs and eventually play a potential role in the cotton stress response.

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