NLRP7 is expressed in the ovine ovary and associated with in vitro pre-implantation embryo development

文献类型： 外文期刊

第一作者： Li, Guangdong

作者： Li, Guangdong;Lv, Dongying;Zhang, Lu;Zhang, Zhenzhen;Wang, Jing;Yang, Minghui;Tao, Jingli;Wu, Hao;Wu, Yingjie;Lian, Zhengxing;Liu, Guoshi;Tian, Xiuzhi;Ma, Teng;Cui, Wei;Cui, Wei

作者机构：

期刊名称：REPRODUCTION （ 影响因子：3.906； 五年影响因子：4.51 ）

ISSN： 1470-1626

年卷期： 2019 年 158 卷 5 期

页码：

收录情况： SCI

摘要： NLRP (NACHT, LRR and PYD domain-containing proteins) family plays pivotal roles in mammalian reproduction. Mutation of NLRP7 is often associated with human recurrent hydatidiform moles. Few studies regarding the functions of NLRP7 have been performed in other mammalian species rather than humans. In the current study, for the first time, the function of NLRP7 has been explored in ovine ovary. NLRP7 protein was mainly located in ovarian follicles and in in vitro pre-implantation embryos. To identify its origin, 763 bp partial CDS of NLRP7 deriving from sheep cumulus oocyte complexes (COCs) was cloned, it showed a great homology with Homo sapiens. The high levels of mRNA and protein of NLRP7 were steadily expressed in oocytes, parthenogenetic embryos or IVF embryos. NLRP7 knockdown by the combination of siRNA and shRNA jeopardized both the parthenogenetic and IVF embryo development. These results strongly suggest that NLRP7 plays an important role in ovine reproduction. The potential mechanisms of NLRP7 will be fully investigated in the future.

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