Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus

文献类型： 外文期刊

第一作者： Yin, Chaomin

作者： Yin, Chaomin;Fan, Xiuzhi;Ma, Kun;Chen, Zheya;Shi, Defang;Yao, Fen;Gao, Hong;Yin, Chaomin;Gao, Hong;Ma, Aimin

作者机构：

关键词： Pleurotus ostreatus; lectin; promoter; Agrobacte-rium tumefaciens; light-induction

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN： 1225-8873

年卷期： 2020 年 58 卷 1 期

页码：

收录情况： SCI

摘要： A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tume-faciens. PCR and Southern blot analyses confirmed the Plp-egfp-hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intra-cellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus.

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