Lateral Flow Assay for Determination of Tebuconazole in Agricultural Products

文献类型： 外文期刊

第一作者： Xu Jun-Li

作者： Xu Jun-Li;Yang Li-Fei;Xu Jun-Li;Liu Bei-Bei;Wang Yu-Long;Li Pan;Yang Kang;Wu Qin;Jiang Lan;Zhang Hao-Ran;Zhang Cun-Zheng;Zhang Cun-Zheng

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关键词： Tebuconazole residue; Immunochromatographic strip; High performance liquid chromatography-tandem mass spectrometry

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2019 年 47 卷 11 期

页码：

收录情况： SCI

摘要： A Lateral flow assay was developed for the rapid determination of Tebuconazole residues in wheat and vegetables based on the high affinity and specific anti-Tebuconazole monoclonal antibody. For the lateral flow assay development, firstly, 20 nm size colloidal gold nanoparticle was synthesized to label anti-Tebuconazole monoclonal antibody (Mab) as a detection agent. Teb-OVA (0.3 mg/mL) conjugate and goat anti-mouse IgG antibody (1 mg/mL) were coated on nitric acid fiber membrane (NC) to form the test line (T) and quality control line (C), respectively. Under the optimized conditions, the assembled immunochromatographic strip could accomplish the detection of Tebuconazole within 15 min. The Limit of detection observed by naked eyes was 6.25 ng/mL, with negligible cross-reactivity to the analogs of Tebuconazole. Meanwhile, for comparison, a high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established to determine Tebuconazole accurately and quantitatively. The results released that these two developed methods matched each other very well. These two methods could be successfully employed for the detection of Tebuconazole in wheat, cucumber, and cabbage , high-throughput, specific and precise. Immunochromatographic strip could contribute to on-site determination, and LC-MS/MS based method provided accurate quantification.

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