Lateral Flow Immunoassay for Simultaneous Determination of Four Kinds of Pyrethroid Pesticides in Vegetables and Fruits

文献类型： 外文期刊

第一作者： Hu Jing

作者： Hu Jing;Liang Xiao;Liu Xian-Jin;Zhang Cun-Zheng;Guo Yi-Rong;Zhu Guo-Nian;Liu Feng-Quan;Wang Ming-Hua;Wang Li-Min;Hua Xiu-De

作者机构：

关键词： Pyrethroid pesticide;Lateral flow immunoassay;Rapid detection

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2016 年 44 卷 12 期

页码：

收录情况： SCI

摘要： A monoclonal antibody based lateral flow immunoassay was developed for the simultaneous determination of four pyrethroid pesticides in vegetables and fruits, including fenpropathrin, cypermethrin, deltamethrin, and cyhalothrin. The colloidal gold particles (20-nm) were prepared by the method of sodium citrate reduction and conjugated with goat anti. mice IgG. Antigen and rabbit anti. goat IgG were immobilized on the nitrocellulose membrane as the test line (T line) and the control line (C line), separately. By sticking on PVC board, a gold immunochromatographic strip was assembled. About 10 g of samples was extracted by acetonitrile, and then diluted four times with PBS buffer. Anti. pyrethroid McAb was added in 100 mu L of this extract solution for the antibody. antigen binding, thereafter, the mixture was ready for the strip testing. The results revealed that the test could be done within 10 min, and the cutoff values reached 0. 5, 5. 0, 5. 0, 5. 0 mu g/mL for fenpropathrin, cypermethrin, deltamethrin, cyhalothrin, respectively. If the extraction going through QuEChERS method, the detection limit of method could be improved 16 times. Thereafter, the lateral flow immunoassay was employed for the verification test with 13 types of vegetables and fruits, except that the result of chillies presented the false positive result, the other results obtained higher consistency with the results of GC test. This new format of test strip using colloidal gold labeled goat anti. mice IgG as tracer, which could give more stable and repeatable results than classic test strip, would provide a new method for the simultaneous determination of four pyrethroid pesticides in vegetables and fruits.

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