Surface display of classical swine fever virus E2 glycoprotein on gram-positive enhancer matrix (GEM) particles via the SpyTag/SpyCatcher system
文献类型： 外文期刊
第一作者： Li, Ding
作者： Li, Ding;Zhang, Haoming;Yang, Li;Chen, Jin;Zhang, Yuanpeng;Yu, Xiaoming;Zheng, Qisheng;Hou, Jibo;Li, Ding;Zhang, Haoming;Yang, Li;Chen, Jin;Zhang, Yuanpeng;Yu, Xiaoming;Zheng, Qisheng;Hou, Jibo;Li, Ding;Zhang, Haoming;Yang, Li;Chen, Jin;Zhang, Yuanpeng;Yu, Xiaoming;Zheng, Qisheng;Hou, Jibo
作者机构：
关键词： Classical swine fever virus E2 protein; Glycosylation; SpyTag/SpyCatcher system; Conjugation; Surface display
期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）
ISSN： 1046-5928
年卷期： 2020 年 167 卷
页码：
收录情况： SCI
摘要： The E2 envelope protein is the main protective antigen of classical swine fever virus (CSFV). Importantly, gram-positive enhancer matrix (GEM) particles can work as an immunostimulant and/or carrier system to improve the immune effect of antigens. In this study, the artificially designed E2-Spy was expressed and glycosylated in Pichia pastoris, and subsequently conjugated with SpyCatcher-PA which was expressed in Escherichia coli. The conjugated E2-Spy-PA was displayed on the surface of GEM particles, generating the E2-Spy-PA-GEM complex. Blocking ELISA analysis and neutralization assays showed that both E2-Spy and E2-Spy-PA-GEM complexes induced high levels of anti-CSFV antibodies in mice. Furthermore, statistical analyses indicated that the E2-Spy-PA-GEM complex exhibited enhanced immunogenicity compared with E2-Spy alone.
分类号：
- 相关文献
作者其他论文 更多>>
-
Bacterial cellulose and photothermal material from biomass-based zero-waste biorefinery functionalized chitosan film for sustainable active food packaging
作者：Yang, Li;Sheng, Wenyang;Yang, Yichen;He, Kaiwen;Jiang, Guangyang;Tian, Yongqiang;Yang, Li;Sheng, Wenyang;Yang, Yichen;He, Kaiwen;Jiang, Guangyang;Tian, Yongqiang;Yang, Ying
关键词：Zero-waste biorefining; Bacterial cellulose; Photothermal material; Chitosan; Antibacterial activity; Distillers' grains
-
New Isocoumarins from an Endophytic Fungal Strain Diaporthe arengae M2 and their Antibacterial Activities
作者：Liu, De-Sui;Ma, Qing-Yun;Yang, Li;Xie, Qing-Yi;Dai, Hao-Fu;Zhao, You-Xing;Liu, De-Sui;Ma, Qing-Yun;Yang, Li;Xie, Qing-Yi;Dai, Hao-Fu;Zhao, You-Xing;Liu, De-Sui;Zhang, Jun-Feng;Wu, You-Gen
关键词：Endophytic fungi;
Diaporthe sp.; Isocoumarin derivatives; Antibacterial activity -
Temperature-controllable antibacterial film mediated by a photoresponsive metal-polyphenol framework for long-term food preservation
作者：Jiang, Guangyang;Sheng, Wenyang;Yang, Li;Yang, Yichen;Tian, Yongqiang;Jiang, Guangyang;Sheng, Wenyang;Yang, Li;Yang, Yichen;Tian, Yongqiang;Tang, Tingting;Wang, Chenzhi
关键词：Cu-CGA nanoparticles; Photothermal; Antimicrobial activity; Food packaging; Cherry tomatoes preservation
-
Transcriptomic Analysis of Wheat Under Multi LED Light Conditions
作者：Sun, Lei;Ma, Chunhong;Jiao, Bo;Wang, Jiao;Zhao, Pu;Dong, Fushuang;Zhou, Shuo;Li, Ding
关键词：mono-light; transcriptome analysis; circadian clock
-
Comprehensive Analysis of Hormonal Signaling Pathways and Gene Expression in Flesh Segment Development of Chinese Bayberry (Myrica rubra)
作者：Fu, Yihan;Yang, Li;Zong, Yu;Li, Yongqiang;Chen, Wenrong;Liao, Fanglei;Guo, Weidong;Zhang, Shuwen;Qi, Xingjiang;Yang, Li;Zong, Yu;Li, Yongqiang;Chen, Wenrong;Liao, Fanglei;Guo, Weidong
关键词：Chinese bayberry; flesh segments; fruit development; endogenous hormones; transcriptome analysis; paraffin sectioning; immunofluorescence localization
-
Crop sample prediction and early mapping based on historical data: Exploration of an explainable FKAN framework
作者：Cheng, Feifei;Qiu, Bingwen;Yang, Peng;Wu, Wenbin;Yu, Qiangyi;Qian, Jianping;Wu, Bingfang;Chen, Jin;Chen, Xuehong;Tubiello, Francesco N.;Tryjanowski, Piotr;Takacs, Viktoria;Duan, Yuanlin;Lin, Lihui;Wang, Laigang;Zhang, Jianyang;Dong, Zhanjie
关键词：Historical Data; Sample generation; Crop mapping; Interpretability; Google Earth Engine
-
A robust framework for mapping complex cropping patterns: The first national-scale 10 m map with 10 crops in China using Sentinel 1/2 images
作者：Qiu, Bingwen;Wu, Fangzheng;Hu, Xiang;Yang, Peng;Wu, Wenbin;Qian, Jianping;Chen, Jin;Chen, Xuehong;He, Liyin;Joe, Berry;Tubiello, Francesco N.;Wang, Laigang
关键词：Cropping patterns mapping; Model generalization; Dual-driven models; Crop diversity; Sentinel-1/2
