Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

文献类型： 外文期刊

第一作者： Li, Changsheng

作者： Li, Changsheng;An, Dong;Wang, Wenqin;Li, Changsheng;Xiang, Xiaoli;Huang, Yongcai;Zhou, Yong;Wang, Qiong;Wu, Yongrui;Xiang, Xiaoli;Huang, Yongcai;Dong, Jiaqiang;Messing, Joachim;Zhao, Chenxi;Liu, Hongjun;Li, Yubin;Du, Chunguang;Larkins, Brian A.

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2020 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04Mb for B73 and 1.48Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F-2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序