Molecular and functional characterization of two DELLA protein-coding genes in litchi

文献类型： 外文期刊

第一作者： Wang, Yi

作者： Wang, Yi;Wei, Yongzan;Dong, Chen;Liu, Liqin;Jue, Dengwei;Shi, Shengyou;Li, Weicai;He, Shae

作者机构：

关键词： Gibberellin; LcGAI; LcRGL1; Arabidopsis; LcGID1

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2020 年 738 卷

页码：

收录情况： SCI

摘要： DELLA proteins are members of the plant-specific GRAS family, acing as negative regulators of plant growth. In this study, we identified two DELLA protein-coding genes in litchi, denoted as LcGAI and LcRGL1. Motif analysis showed that LcGAI and LcRGL1 proteins both contain a conserved DELLA and TVHYNP motif at the N-terminus as well as LHR1, VHIID, LHR2, PFYRE, and SAW motifs at the C terminus. The fused proteins of LcGAI-GFP and LcRGL1-GFP were both localized in the nucleus. Overexpression of LcGAI and LcRGL1 in Arabidopsis substantially inhibits leaf growth. Expression analysis showed that HLH factors, PRE1 and PRES, were restrained, whereas gibberellin (GA) receptors GIDla and LcGID1b were enhanced in LcGAI and LcRGL1 overexpression lines. Results of the yeast two-hybrid assay showed that LcGAI and LcRGL1 interact with LcGID1b/LcGID1c in a GA dose-dependent manner, whereas LcGAI and LcRGL1 had a greater binding capacity to LcGID1b than LcGID1c. These observations suggested that LcGAI and LcRGL1 proteins are nuclear growth repressors.

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