Preparation and structural characterization of acid-extracted polysaccharide from Grifola frondosa and antitumor activity on S180 tumor-bearing mice

文献类型： 外文期刊

第一作者： Zhang, Haibo

作者： Zhang, Haibo;Yu, Juan;Liu, Anjun;Dong, Xiaodan;Ji, Haiyu;Yu, Juan;Liu, Anjun

作者机构：

关键词： Acid-extracted polysaccharide; G; frondosa; Antitumor activities

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 234 卷

页码：

收录情况： SCI

摘要： In this study, an acid-extracted polysaccharide (GFP-A) was extracted from the fruiting bodies of G. frondosa with 1 % hydrochloric acid solution. Our study aimed to imitate the processes of digestion, absorption and antitumor activities of polysaccharides from G. frondosa under the acid environment of stomach in the body. The pre-liminary structural analysis resulted that GFP-A (about 1.10 symbolscript 106 Da) was a neutral polysaccharide composed of xylose, mannose, glucose (molar ratio: 0.12:1.00:6.98) with alpha-type glycosidic linkages. Additionally, antitumor activities on S180 tumor-bearing mice showed that GFP-A could effectively inhibit the growth of S180 tumor cells by protecting immune organs (thymus and spleen), activating immune cells (NK cells, lymphocytes and macrophages), upregulating the secretion of serum cytokines (TNF-alpha, IL-2 and IFN-gamma) in vivo. H & E staining and cell cycle determination further demonstrated that GFP-A could induce S180 tumor cells apoptosis via arresting them in G1 phase. These results demonstrated that GFP-A could provide a theoretical basis for treatment of cancer.

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