Pyramiding of cry toxins and methanol producing genes to increase insect resistance in cotton

文献类型： 外文期刊

第一作者： Razzaq, Abdul

作者： Razzaq, Abdul;Zafar, Muhammad Mubashar;Deng Xiaoying;Ge Qun;Ren, Maozhi;Gong, Wankui;Yuan Youlu;Ali, Arfan;Nawaz, Aisha;Li Pengtao;Ashraf, Muhammad

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关键词： Cotton; transformation; eagle-2; agrobacterium; pectin methyl esterase enzyme; insecticidal cry proteins

期刊名称：GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN （ 影响因子：3.074； 五年影响因子：4.585 ）

ISSN： 2164-5698

年卷期： 2021 年 12 卷 1 期

页码：

收录情况： SCI

摘要： The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression of cry genes from Bacillus thuringiensis as a strategy to improve insect pest control in cotton is presented. We constructed a cassette containing two cry genes (cry1Fa and Cry32Aa) and two pme genes, one from Arabidopsis thaliana (AtPME), and other from Aspergillus. niger (AnPME) in pCAMBIA1301 plant expression vector using CAMV-35S promoter. This construction was transformed in Eagle-2 cotton variety by using shoot apex-cut Agrobacterium-mediated transformation. Expression of cry genes and pme genes was confirmed by qPCR. Methanol production was measured in control and in the cry and pme transformed plants showing methanol production only in transformed plants, in contrast to the non-transgenic cotton plants. Finally, insect bioassays performed with transgenic plants expressing cry and pme genes showed 100% mortality for Helicoverpa armigera (cotton bollworm) larvae, 70% mortality for Pectinophora gossypiella (pink bollworm) larvae and 95% mortality of Earias fabia, (spotted bollworm) larvae, that was higher than the transgenic plants expressing only cry genes that showed 84%, 49% and 79% mortality, respectively. These results demonstrate that Bt. cry-genes coupled with pme genes are an effective strategy to improve the control of different insect pests.

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